Source:http://linkedlifedata.com/resource/pubmed/id/15979177
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2006-1-23
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pubmed:abstractText |
A semi-selective medium, PA 20, was developed for the isolation and enumeration of Pantoea ananatis from plant material, specifically from onion seed. The medium has a pH of 8.0 and contains NH4H2PO4, K2HPO4, magnesium sulphate, NaCl, d (+) arabitol, crystal violet, bromothymol blue and thallium nitrate. All P. ananatis strains from a variety of hosts produced characteristic yellow colonies in 6-7 days at 25 degrees C. Plating efficiencies on PA 20 in comparison to nutrient agar ranged from 92 to 112%. Recovery from naturally infested and artificially contaminated onion seed was high, with an almost total reduction of saprophytes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0167-7012
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading | |
pubmed:year |
2006
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pubmed:articleTitle |
PA 20, a semi-selective medium for isolation and enumeration of Pantoea ananatis.
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pubmed:affiliation |
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa. goszczynskat@arc.agric.za
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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