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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-6-24
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pubmed:abstractText |
GABA(A) receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABA(A) receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DDX47 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/GABARAP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15977068-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:15977068-Apoptosis,
pubmed-meshheading:15977068-Carrier Proteins,
pubmed-meshheading:15977068-Cell Line, Tumor,
pubmed-meshheading:15977068-Chromatography, Liquid,
pubmed-meshheading:15977068-DEAD-box RNA Helicases,
pubmed-meshheading:15977068-DNA, Complementary,
pubmed-meshheading:15977068-Glutathione Transferase,
pubmed-meshheading:15977068-Humans,
pubmed-meshheading:15977068-Immunoprecipitation,
pubmed-meshheading:15977068-Mass Spectrometry,
pubmed-meshheading:15977068-Microtubule-Associated Proteins,
pubmed-meshheading:15977068-Neurons,
pubmed-meshheading:15977068-Protein Binding,
pubmed-meshheading:15977068-RNA Helicases,
pubmed-meshheading:15977068-Recombinant Fusion Proteins,
pubmed-meshheading:15977068-Tetrazolium Salts,
pubmed-meshheading:15977068-Thiazoles,
pubmed-meshheading:15977068-Two-Hybrid System Techniques
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pubmed:year |
2005
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pubmed:articleTitle |
GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47).
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pubmed:affiliation |
Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Keumam Dong, Dukjin Ku, Jeonju, 561-712, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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