Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-6-23
pubmed:abstractText
Salmonella enterica serovar Typhimurium utilizes macrophages to disseminate from the intestine to deeper tissues within the body. While S. enterica serovar Typhimurium has been shown to kill its host macrophage, it can persist intracellularly beyond 18 h postinfection. To identify factors involved in late stages of infection, we screened a transposon library made in S. enterica serovar Typhimurium for the ability to persist in J774 macrophages at 24 h postinfection. Through this screen, we identified a gene, sciS, found to be homologous to icmF in Legionella pneumophila. icmF, which is required for intracellular multiplication, is conserved in several gram-negative pathogens, and its homolog appears to have been acquired horizontally in S. enterica serovar Typhimurium. We found that an sciS mutant displayed increased intracellular numbers in J774 macrophages when compared to the wild-type strain at 24 h postinfection. sciS was maximally transcribed at 27 h postinfection and is repressed by SsrB, an activator of genes required for promoting intracellular survival. Finally, we demonstrate that an sciS mutant is hypervirulent in mice when administered intragastrically. Taken together, these data indicate a role for SciS in controlling intracellular bacterial levels at later stages of infection and attenuating virulence in a murine host.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10051653, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10209748, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10348855, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10417651, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10499590, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10698741, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10844662, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10981695, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-10992474, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11029688, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11133955, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11157927, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11488817, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11553591, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11696033, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11886586, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-11994420, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12127983, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12180921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12193612, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12437215, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12441401, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12721316, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12950921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-12954091, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-14636560, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-15155646, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-15158775, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-15385501, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-15385502, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-15469431, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-1686293, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-2840333, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-8633040, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-8790417, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-8885278, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9294441, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9465074, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9573114, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9630225, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9786194, http://linkedlifedata.com/resource/pubmed/commentcorrection/15972528-9864218
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4338-45
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
sciS, an icmF homolog in Salmonella enterica serovar Typhimurium, limits intracellular replication and decreases virulence.
pubmed:affiliation
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd., L220, Portland, Oregon 97201, USA. parsonsd@ohsu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural