| pubmed-article:15967165 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C1622501 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C0037907 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C0120465 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C1419227 | lld:lifeskim |
| pubmed-article:15967165 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:15967165 | pubmed:issue | 1-4 | lld:pubmed |
| pubmed-article:15967165 | pubmed:dateCreated | 2005-6-21 | lld:pubmed |
| pubmed-article:15967165 | pubmed:abstractText | Endothelial cell (EC) migration has an important role in angiogenesis. Sphingosine-1 phosphate (S1P) stimulates EC migration via activation of Gi proteins. In this study, we characterized a mouse guanine nucleotide exchange factor (GEF) P-Rex2b for its regulation by Gbetagamma and PI3K and its role in S1P-induced Rac1 activation and cell migration in ECs. We found that co-expression of Gbetagamma or an active form of PI3K (PI3K(AC)) with P-Rex2b increased the SRE. Luciferase (SRE.L) reporter gene activity that can be stimulated by the Rho family of small GTPases including Rac1. Co-expression with P-Rex2b of Gbetagamma and PI3K(AC) or wild type PI3Kgamma that can be activated by Gbetagamma led to further increases in the reporter gene activity. Together with the finding that co-expression of Gbetagamma and/or PI3K(AC) increased the levels of active Rac1, we conclude that P-Rex2b is a Rac GEF that can be regulated by Gbetagamma and PI3K. Additionally, we demonstrated that Gbetagamma interacted with P-Rex2b, probably through P-Rex2b sequences at the PH domain and that the DEP and PDZ domains of P-Rex2b exerted an inhibitory effect on P-Rex2b's activity because their deletion increased the SER.L reporter gene activity. Furthermore, we found that P-Rex2b is involved in S1P-induced Rac1 activation and cell migration in ECs because siRNA-mediated suppression of P-Rex2b expression in ECs-diminished Rac1 activation and cell migration in response to S1P. Therefore, P-Rex2b is a physiologically significant Rac1 GEF that has an important role in the regulation of EC migration. | lld:pubmed |
| pubmed-article:15967165 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15967165 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15967165 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15967165 | pubmed:month | May | lld:pubmed |
| pubmed-article:15967165 | pubmed:issn | 1098-8823 | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:WuDianqingD | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:ZhongLiL | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:PaikJi-HyeJH | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:WangZhenglong... | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:HlaTimT | lld:pubmed |
| pubmed-article:15967165 | pubmed:author | pubmed-author:PaikJie-HyeJH | lld:pubmed |
| pubmed-article:15967165 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15967165 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:15967165 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15967165 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15967165 | pubmed:pagination | 95-104 | lld:pubmed |
| pubmed-article:15967165 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:15967165 | pubmed:meshHeading | pubmed-meshheading:15967165... | lld:pubmed |
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| pubmed-article:15967165 | pubmed:meshHeading | pubmed-meshheading:15967165... | lld:pubmed |
| pubmed-article:15967165 | pubmed:meshHeading | pubmed-meshheading:15967165... | lld:pubmed |
| pubmed-article:15967165 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15967165 | pubmed:articleTitle | Role of guanine nucleotide exchange factor P-Rex-2b in sphingosine 1-phosphate-induced Rac1 activation and cell migration in endothelial cells. | lld:pubmed |
| pubmed-article:15967165 | pubmed:affiliation | Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, 06030, USA. zli@neuron.uchu.edu | lld:pubmed |
| pubmed-article:15967165 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15967165 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15967165 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15967165 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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