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rdf:type |
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lifeskim:mentions |
umls-concept:C0001473,
umls-concept:C0007589,
umls-concept:C0018183,
umls-concept:C0439677,
umls-concept:C1167087,
umls-concept:C1333030,
umls-concept:C1335840,
umls-concept:C1335843,
umls-concept:C1511938,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1548534,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1711351
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pubmed:issue |
1
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pubmed:dateCreated |
2005-11-3
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pubmed:abstractText |
Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a defective Brg1 protein negates the normal activity of the SWI/SNF complex and delays or blocks differentiation. To investigate the role of SWI/SNF complexes during myelopoiesis, we stably expressed a dominant negative (dn) Brg1 mutant in the myeloid lineage. Forced expression of dnBrg1 in IL-3-dependent murine 32Dcl3 myeloid progenitor cells results in a profound delay in the granulocyte-colony stimulating factor (G-CSF) induced granulocytic maturation. These cells also exhibit a significant decrease in the expression of both CD11b and Gr-1 surface receptors, which are normally upregulated during granulopoiesis, and show sustained expression of myeloperoxidase, which is synthesized primarily during the promyelocytic (blast) stage of myeloid development. Thus, dnBrg1 expression causes a developmental block at the promyelocytic/metamyelocytic stage of myeloid differentiation. Our findings indicate that the normal chromatin remodeling function of Brg1 is necessary for the G-CSF dependent differentiation of myeloid cells towards the granulocytic lineage. This dependency on Brg1 may reflect a stringent requirement for chromatin remodeling at a critical stage of hematopoietic cell maturation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9541
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2005 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
206
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
112-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15965950-Adenosine Triphosphatases,
pubmed-meshheading:15965950-Animals,
pubmed-meshheading:15965950-Biological Markers,
pubmed-meshheading:15965950-Cell Differentiation,
pubmed-meshheading:15965950-Cell Line,
pubmed-meshheading:15965950-Cell Lineage,
pubmed-meshheading:15965950-DNA Helicases,
pubmed-meshheading:15965950-Granulocytes,
pubmed-meshheading:15965950-Macromolecular Substances,
pubmed-meshheading:15965950-Mice,
pubmed-meshheading:15965950-Myeloid Cells,
pubmed-meshheading:15965950-Nuclear Proteins,
pubmed-meshheading:15965950-Protein Subunits,
pubmed-meshheading:15965950-Stem Cells,
pubmed-meshheading:15965950-Transcription Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for myeloid differentiation to granulocytes.
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pubmed:affiliation |
Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0106, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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