Source:http://linkedlifedata.com/resource/pubmed/id/15964765
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2005-7-11
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| pubmed:abstractText |
Three tandemly arrayed protocadherin gene clusters (Pcdh-alpha, -beta, -gamma) comprising more than 50 genes are found in human and mouse. Here, we have investigated the expression and distribution of individual gamma-protocadherins (Pcdhs-gamma) in the developing mouse brain. We find that transfection of Pcdh-gamma genes promotes calcium-dependent cell adhesion in HEK 293 cells. Furthermore, Pcdh-gamma can be recruited to synapses of transfected primary hippocampal neurons. Several individual members of the in total 22 Pcdhs-gamma were chosen to examine the expression of the three subfamilies, Pcdh-gammaA, -gammaB, and -gammaC. These Pcdh-gamma transcripts are expressed all over the brain, with minor regional and cell-type specific differences. Interestingly, a distinct, later onset of expression is observed for Pcdh-gammaC5, a gene located at the end of the Pcdh-gamma cluster. Largely overlapping expression patterns of individual Pcdh-gamma proteins are detected with anti-peptide antibodies. Small differences are observed in the staining of dendritic processes and synapse-rich layers. Our results support the idea that Pcdhs-gamma participate in neuronal differentiation and may be implicated in the fine-tuning of neuronal morphology and synaptogenesis. Cell autonomous regulation of transcription might generate the widespread distribution of individual Pcdhs-gamma in the brain, which is strikingly different from the restricted expression patterns observed for classical cadherins. Thus, a defined set of Pcdhs-gamma may engage in neuronal adhesion and signaling on the cellular level.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1044-7431
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
29
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
603-16
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15964765-Animals,
pubmed-meshheading:15964765-Animals, Newborn,
pubmed-meshheading:15964765-Brain,
pubmed-meshheading:15964765-Cadherins,
pubmed-meshheading:15964765-Cell Adhesion,
pubmed-meshheading:15964765-Cell Differentiation,
pubmed-meshheading:15964765-Cell Line,
pubmed-meshheading:15964765-Cells, Cultured,
pubmed-meshheading:15964765-Dendrites,
pubmed-meshheading:15964765-Gene Expression Regulation, Developmental,
pubmed-meshheading:15964765-Humans,
pubmed-meshheading:15964765-Mice,
pubmed-meshheading:15964765-Neurons,
pubmed-meshheading:15964765-Protein Isoforms,
pubmed-meshheading:15964765-Rats,
pubmed-meshheading:15964765-Rats, Sprague-Dawley,
pubmed-meshheading:15964765-Signal Transduction,
pubmed-meshheading:15964765-Synapses,
pubmed-meshheading:15964765-Transfection
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Differential expression of individual gamma-protocadherins during mouse brain development.
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| pubmed:affiliation |
Department of Molecular Embryology, Max-Planck Institute of Immunobiology, D-79108 Freiburg, Germany. frank@immunbio.mpg.de
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|