Linked Life Data

15964425

Source:http://linkedlifedata.com/resource/pubmed/id/15964425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-6-20
pubmed:abstractText
Acute allograft rejection is driven by production of cytokines such as interleukin(IL)-2 that activate and expand alloreactive T cells by ligating high-affinity IL-2alpha receptors. Lipid rafts on the membranes of T cells may be the functional microdomains for IL-2alpha receptor. Flow cytometric analysis was performed for expression of CD25 (IL-2alpha receptor) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Ralpha in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Cells were stimulated with IL-2 and expression of CD25 (IL-2alpha receptor) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Ralpha was localized in lipid rafts. Lipid rafts are the functional microdomains for IL-2alpha receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0041-1345
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2395-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Interleukin-2alpha receptor in membrane lipid rafts.
pubmed:affiliation
Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, People's Republic of China. liqiurong@yahoo.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't