Source:http://linkedlifedata.com/resource/pubmed/id/15963260
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2005-6-20
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pubmed:abstractText |
Previous reports describing Cajal-like interstitial cells in human uterus are contradictory in terms of c-kit immunoreactivity: either negative (but vimentin-positive) in pregnant myometrium, or positive, presumably in the endometrium. The aim of this study was to verify the existence of human myometrial Cajal-like interstitial cells (m-CLIC). Six different, complementary approaches were used: 1) methylene-blue supravital staining of tissue samples (cryosections), 2) methylene blue and Janus green B vital staining (m-CLIC and mitochondrial markers, respectively), and 3) extracellular single-unit electrophysiological recordings in cell cultures, 4) non-conventional light microscopy on glutaraldehyde/osmium fixed, Epon-embedded semi-thin sections (less than 1 microm) stained with toluidine blue (TSM), 5) transmission electron microscopy (TEM), and 6) immunofluorescence (IF). We found m-CLIC in myometrial cryosections and in cell cultures. In vitro, m-CLIC represented approximately 7% of the total cell number. m-CLIC had 2-3 characteristic processes which were very long (approximately 60 microm), very thin (< or =0.5 microm) and moniliform. The dilated portions of processes usually accommodated mitochondria. In vitro, m-CLIC exhibited spontaneous electrical activity (62.4+/-7.22 mV membrane potentials, short duration: 1.197+/-0.04 ms). Moreover, m-CLIC fulfilled the usual TEM criteria, the so-called 'gold' or 'platinum' standards (e.g. the presence of discontinuous basal lamina, caveolae, endoplasmic reticulum, and close contacts between each other, with myocytes, nerve fibers and/or capillaries etc.). IF showed that m-CLIC express CD117/c-kit, sometimes associated with CD34, with vimentin along their processes. In conclusion, we describe myometrial Cajal-like interstitial cells that have affinity for methylene blue and Janus green B vital dyes, fulfill (all) TEM criteria, express CD117/c-kit and have spontaneous electric activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Methylene Blue,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Vimentin
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pubmed:status |
MEDLINE
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pubmed:issn |
1582-1838
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pubmed:author |
pubmed-author:BragaR IRI,
pubmed-author:CeafalanLauraL,
pubmed-author:CionteaSanda MSM,
pubmed-author:CretoiuDD,
pubmed-author:GherghiceanuMihaelaM,
pubmed-author:HinescuM EME,
pubmed-author:MalincencoMarianaM,
pubmed-author:PopescuL MLM,
pubmed-author:RaduEE,
pubmed-author:RegaliaTT,
pubmed-author:ZagreanLL
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
407-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15963260-Actins,
pubmed-meshheading:15963260-Antigens, CD34,
pubmed-meshheading:15963260-Cells, Cultured,
pubmed-meshheading:15963260-Connective Tissue Cells,
pubmed-meshheading:15963260-Electrophysiology,
pubmed-meshheading:15963260-Female,
pubmed-meshheading:15963260-Fluorescent Antibody Technique,
pubmed-meshheading:15963260-Gap Junctions,
pubmed-meshheading:15963260-Humans,
pubmed-meshheading:15963260-Methylene Blue,
pubmed-meshheading:15963260-Microscopy, Electron, Transmission,
pubmed-meshheading:15963260-Microscopy, Fluorescence,
pubmed-meshheading:15963260-Myocytes, Smooth Muscle,
pubmed-meshheading:15963260-Myometrium,
pubmed-meshheading:15963260-Pregnancy,
pubmed-meshheading:15963260-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:15963260-Vimentin
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pubmed:articleTitle |
C-kit immunopositive interstitial cells (Cajal-type) in human myometrium.
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pubmed:affiliation |
Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania. sciontea@univermed-cdgm.ro
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pubmed:publicationType |
Journal Article
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