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pubmed-article:15956020pubmed:abstractTextA serial study was performed to develop a protein-embedding technique for standardization of immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. A protein carrier matrix must have two phases, a liquid phase to allow uniform mixing of a protein and a solid phase forming a 'block' that can be fixed and processed in the same manner as human tissue. This standardized protein block would serve as a source of thin sections for control of IHC and therefore must also withstand the boiling conditions of antigen retrieval (AR). After multiple experiments, a method was developed utilizing polymer microsphere (beads) as a support medium for protein. The method showed particular promise for quantitative IHC.lld:pubmed
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pubmed-article:15956020pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:15956020pubmed:articleTitleProtein-embedding technique: a potential approach to standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections.lld:pubmed
pubmed-article:15956020pubmed:affiliationDepartment of Pathology, University of Southern California Keck School of Medicine, HMR 204, 2011 Zonal Avenue, Los Angeles, CA 90033, USA.lld:pubmed
pubmed-article:15956020pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15956020pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:15956020pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed
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