Linked Life Data

15951111

Source:http://linkedlifedata.com/resource/pubmed/id/15951111

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2005-7-11
pubmed:abstractText
Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of T. gondii for infection in humans. In the present study, we report the first isolation of viable T. gondii from finishing pigs in Brazil. Antibodies to T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically T. gondii isolates from pigs from Brazil are distinct from isolates of T. gondii from pigs in the USA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0304-4017
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-11
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil.
pubmed:affiliation
Departamento de Medicina Veterinária Preventiva, Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal, SP, Brazil.
pubmed:publicationType
Journal Article