| pubmed-article:15950768 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C2745888 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C1709016 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C1882932 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:15950768 | lifeskim:mentions | umls-concept:C1514861 | lld:lifeskim |
| pubmed-article:15950768 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:15950768 | pubmed:dateCreated | 2005-6-13 | lld:pubmed |
| pubmed-article:15950768 | pubmed:abstractText | Despite the success and popularity of microarrays as a high-throughput technology for gene-expression studies, its sensitivity is as yet fairly limited. We have successfully combined the use of PCR-Select cDNA subtraction and Affymetrix GeneChips (AGC) to identify differentially expressed gene markers. Total RNA (totRNA) from combined hippocampus and cerebellum tissues of 2-week-old rat pups maintained for 5 weeks on an n-3 fatty acid (FA) deficient diet supplied to dams was isolated, SMART-amplified, and used for PCR-Select subtraction versus an adequately fed control litter preparation. Subtracted and amplified ds-cDNA end products were fragmented, terminally labeled with biotin-ddUTP and hybridized with RN-U34A AGC. At least 10-fold more potential gene markers with log2(T/D) > or = 1.4 were found versus the traditional AGC technology when the same chip was tested using nonsubtracted targets. Of this set of markers, 30% were robustly validated by real-time relative RT-PCR (rtrRT-PCR) and grouped as "confirmed" markers while the remaining were ascribed as "latent" markers. An improved and universal protocol to provide a rapid assessment for gene profiling in biological specimens is indicated. | lld:pubmed |
| pubmed-article:15950768 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15950768 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15950768 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15950768 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15950768 | pubmed:issn | 0169-328X | lld:pubmed |
| pubmed-article:15950768 | pubmed:author | pubmed-author:YavinEE | lld:pubmed |
| pubmed-article:15950768 | pubmed:author | pubmed-author:SabanSS | lld:pubmed |
| pubmed-article:15950768 | pubmed:author | pubmed-author:KupersteinFF | lld:pubmed |
| pubmed-article:15950768 | pubmed:author | pubmed-author:YakubovEE | lld:pubmed |
| pubmed-article:15950768 | pubmed:author | pubmed-author:DinermanPP | lld:pubmed |
| pubmed-article:15950768 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15950768 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:15950768 | pubmed:volume | 137 | lld:pubmed |
| pubmed-article:15950768 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15950768 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15950768 | pubmed:pagination | 110-8 | lld:pubmed |
| pubmed-article:15950768 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:15950768 | pubmed:meshHeading | pubmed-meshheading:15950768... | lld:pubmed |
| pubmed-article:15950768 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15950768 | pubmed:articleTitle | Improved representation of gene markers on microarray by PCR-Select subtracted cDNA targets. | lld:pubmed |
| pubmed-article:15950768 | pubmed:affiliation | Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel. | lld:pubmed |
| pubmed-article:15950768 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15950768 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15950768 | pubmed:publicationType | Validation Studies | lld:pubmed |
