Source:http://linkedlifedata.com/resource/pubmed/id/15950768
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2005-6-13
|
| pubmed:abstractText |
Despite the success and popularity of microarrays as a high-throughput technology for gene-expression studies, its sensitivity is as yet fairly limited. We have successfully combined the use of PCR-Select cDNA subtraction and Affymetrix GeneChips (AGC) to identify differentially expressed gene markers. Total RNA (totRNA) from combined hippocampus and cerebellum tissues of 2-week-old rat pups maintained for 5 weeks on an n-3 fatty acid (FA) deficient diet supplied to dams was isolated, SMART-amplified, and used for PCR-Select subtraction versus an adequately fed control litter preparation. Subtracted and amplified ds-cDNA end products were fragmented, terminally labeled with biotin-ddUTP and hybridized with RN-U34A AGC. At least 10-fold more potential gene markers with log2(T/D) > or = 1.4 were found versus the traditional AGC technology when the same chip was tested using nonsubtracted targets. Of this set of markers, 30% were robustly validated by real-time relative RT-PCR (rtrRT-PCR) and grouped as "confirmed" markers while the remaining were ascribed as "latent" markers. An improved and universal protocol to provide a rapid assessment for gene profiling in biological specimens is indicated.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Omega-3,
http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0169-328X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
13
|
| pubmed:volume |
137
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
110-8
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15950768-Animals,
pubmed-meshheading:15950768-Animals, Newborn,
pubmed-meshheading:15950768-Brain Chemistry,
pubmed-meshheading:15950768-Cerebellum,
pubmed-meshheading:15950768-DNA, Complementary,
pubmed-meshheading:15950768-Fatty Acids, Omega-3,
pubmed-meshheading:15950768-Food, Formulated,
pubmed-meshheading:15950768-Gene Expression Profiling,
pubmed-meshheading:15950768-Gene Expression Regulation,
pubmed-meshheading:15950768-Genetic Markers,
pubmed-meshheading:15950768-Hippocampus,
pubmed-meshheading:15950768-Lipids,
pubmed-meshheading:15950768-Nerve Tissue Proteins,
pubmed-meshheading:15950768-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15950768-Polymerase Chain Reaction,
pubmed-meshheading:15950768-RNA, Messenger,
pubmed-meshheading:15950768-Rats,
pubmed-meshheading:15950768-Rats, Sprague-Dawley,
pubmed-meshheading:15950768-Stress, Physiological
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Improved representation of gene markers on microarray by PCR-Select subtracted cDNA targets.
|
| pubmed:affiliation |
Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Validation Studies
|