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rdf:type |
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lifeskim:mentions |
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0162326,
umls-concept:C0205245,
umls-concept:C0220905,
umls-concept:C0936012,
umls-concept:C1283195,
umls-concept:C1514562,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
11
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pubmed:dateCreated |
2005-6-13
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pubmed:abstractText |
The lambda5 and VpreB genes encode the components of the surrogate light-chain which forms part of the pre-B cell receptor and plays a key role in B cell development. In the mouse, the lambda5 and VpreB1 genes are closely linked and are co-regulated by a multi-component locus control region. To identify the sequences that regulate lambda5 and VpreB1 expression during B cell development, we have comprehensively mapped the DNaseI hypersensitive sites (HS) in the lambda5-VpreB1 functional domain. The active domain contains 12 HS that are distributed at high density across the 18.3 kb region that forms the lambda5 and VpreB1 functional unit. Analysis of a reporter gene driven by the VpreB1 promoter in transgenic mice identified a novel enhancer associated with two HS located upstream of lambda5. The lambda5-VpreB1 locus was also found to be closely linked to the ubiquitously expressed Topoisomerase-3beta (Topo3beta) gene. The VpreB1 and Topo3beta genes have entirely different expression patterns despite the fact that the two promoters are separated by a distance of only 1.5 kb.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Light Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Light Chains...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pre-B Cell Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1283-92
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15950724-Animals,
pubmed-meshheading:15950724-B-Lymphocytes,
pubmed-meshheading:15950724-Base Sequence,
pubmed-meshheading:15950724-Cell Differentiation,
pubmed-meshheading:15950724-Cell Line,
pubmed-meshheading:15950724-Chromosome Mapping,
pubmed-meshheading:15950724-DNA, Complementary,
pubmed-meshheading:15950724-Deoxyribonuclease I,
pubmed-meshheading:15950724-Enhancer Elements, Genetic,
pubmed-meshheading:15950724-Genes, Regulator,
pubmed-meshheading:15950724-Immunoglobulin Light Chains,
pubmed-meshheading:15950724-Immunoglobulin Light Chains, Surrogate,
pubmed-meshheading:15950724-Immunoglobulin Variable Region,
pubmed-meshheading:15950724-Membrane Glycoproteins,
pubmed-meshheading:15950724-Mice,
pubmed-meshheading:15950724-Mice, Transgenic,
pubmed-meshheading:15950724-Plasmids,
pubmed-meshheading:15950724-Pre-B Cell Receptors,
pubmed-meshheading:15950724-Promoter Regions, Genetic,
pubmed-meshheading:15950724-Receptors, Antigen, B-Cell
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pubmed:year |
2005
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pubmed:articleTitle |
Mapping and functional analysis of regulatory sequences in the mouse lambda5-VpreB1 domain.
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pubmed:affiliation |
Gene regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Campus, Du Cane Road, London W12 ONN, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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