Source:http://linkedlifedata.com/resource/pubmed/id/15950277
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
33
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| pubmed:dateCreated |
2005-7-26
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| pubmed:abstractText |
Articular cartilage has limited intrinsic repair capacity. In order to promote cartilage repair, the amplification and transfer of autologous chondrocytes using three-dimensional scaffolds have been proposed. We have developed an injectable and self-setting hydrogel consisting of hydroxypropyl methylcellulose grafted with silanol groups (Si-HPMC). The aim of the present work is to assess both the in vitro cytocompatibility of this hydrogel and its ability to maintain a chondrocyte-specific phenotype. Primary chondrocytes isolated from rabbit articular cartilage (RAC) and two human chondrocytic cell lines (SW1353 and C28/I2) were cultured into the hydrogel. Methyl tetrazolium salt (MTS) assay and cell counting indicated that Si-HPMC hydrogel did not affect respectively chondrocyte viability and proliferation. Fluorescent microscopic observations of RAC and C28/I2 chondrocytes double-labeled with cell tracker green and ethidium homodimer-1 revealed that chondrocytes proliferated within Si-HPMC. Phenotypic analysis (RT-PCR and Alcian blue staining) indicates that chondrocytes, when three-dimensionnally cultured within Si-HPMC, expressed transcripts encoding type II collagen and aggrecan and produced sulfated glycosaminoglycans. These results show that Si-HPMC allows the growth of differentiated chondrocytes. Si-HPMC therefore appears as a potential scaffold for three-dimensional amplification and transfer of chondrocytes in cartilage tissue engineering.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosaminoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogel,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogels,
http://linkedlifedata.com/resource/pubmed/chemical/Methylcellulose,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Silanes,
http://linkedlifedata.com/resource/pubmed/chemical/hypromellose
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0142-9612
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
26
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6643-51
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15950277-Animals,
pubmed-meshheading:15950277-Biocompatible Materials,
pubmed-meshheading:15950277-Cartilage,
pubmed-meshheading:15950277-Cartilage, Articular,
pubmed-meshheading:15950277-Cell Proliferation,
pubmed-meshheading:15950277-Cell Survival,
pubmed-meshheading:15950277-Cells, Cultured,
pubmed-meshheading:15950277-Chondrocytes,
pubmed-meshheading:15950277-Dimerization,
pubmed-meshheading:15950277-Glycosaminoglycans,
pubmed-meshheading:15950277-Humans,
pubmed-meshheading:15950277-Hydrogel,
pubmed-meshheading:15950277-Hydrogels,
pubmed-meshheading:15950277-Methylcellulose,
pubmed-meshheading:15950277-Microscopy, Fluorescence,
pubmed-meshheading:15950277-Phenotype,
pubmed-meshheading:15950277-Polymerase Chain Reaction,
pubmed-meshheading:15950277-RNA, Messenger,
pubmed-meshheading:15950277-Rabbits,
pubmed-meshheading:15950277-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15950277-Silanes,
pubmed-meshheading:15950277-Time Factors,
pubmed-meshheading:15950277-Tissue Engineering
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| pubmed:year |
2005
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| pubmed:articleTitle |
A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.
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| pubmed:affiliation |
INSERM EM 9903, Research Center on Materials with Biological Interest, School of Dental Surgery, University of Nantes, 1-Place Alexis Ricordeau 44042, Nantes Cedex 1, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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