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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-6-13
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pubmed:abstractText |
There is growing evidence that high levels of the macular xanthophyll carotenoids lutein and zeaxanthin may be protective against visual loss due to age-related macular degeneration, but the actual mechanisms of their protective effects are still poorly understood. We have recently purified, identified and characterized a pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eye which specifically and saturably binds to the two forms of zeaxanthin endogenously found in the foveal region. In this report, we studied the synergistic antioxidant role of zeaxanthin and GSTP1 in egg yolk phosphatidylcholine (EYPC) liposomes using hydrophilic 2,2'-azobis(2-methyl-propionamidine) dihydrochloride (AAPH) and lipophilic 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) as lipid peroxyl radical generators. The two zeaxanthin diastereomers displayed synergistic antioxidant effects against both azo lipid peroxyl radical generators when bound to GSTP1. In the presence of GSTP1, nondietary (3R,3'S-meso)-zeaxanthin was observed to be a better antioxidant than dietary (3R,3'R)-zeaxanthin. This effect was found to be independent of the presence of glutathione. Carotenoid degradation profiles indicated that the zeaxanthin diastereomers in association with GSTP1 were more resistant to degradation which may account for the synergistic antioxidant effects.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2'-azobis(2,4-dimethylvaleronitril...,
http://linkedlifedata.com/resource/pubmed/chemical/2,2'-azobis(2-amidinopropane),
http://linkedlifedata.com/resource/pubmed/chemical/Amidines,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Azo Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/GSTP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione S-Transferase pi,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthophylls,
http://linkedlifedata.com/resource/pubmed/chemical/beta Carotene,
http://linkedlifedata.com/resource/pubmed/chemical/zeaxanthin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
1740
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
116-21
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15949677-Amidines,
pubmed-meshheading:15949677-Antioxidants,
pubmed-meshheading:15949677-Azo Compounds,
pubmed-meshheading:15949677-Drug Stability,
pubmed-meshheading:15949677-Glutathione,
pubmed-meshheading:15949677-Glutathione S-Transferase pi,
pubmed-meshheading:15949677-Glutathione Transferase,
pubmed-meshheading:15949677-Humans,
pubmed-meshheading:15949677-Isoenzymes,
pubmed-meshheading:15949677-Lipid Peroxidation,
pubmed-meshheading:15949677-Liposomes,
pubmed-meshheading:15949677-Nitriles,
pubmed-meshheading:15949677-Oxidants,
pubmed-meshheading:15949677-Phosphatidylcholines,
pubmed-meshheading:15949677-Retina,
pubmed-meshheading:15949677-Stereoisomerism,
pubmed-meshheading:15949677-Xanthophylls,
pubmed-meshheading:15949677-beta Carotene
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pubmed:year |
2005
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pubmed:articleTitle |
Synergistic effects of zeaxanthin and its binding protein in the prevention of lipid membrane oxidation.
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pubmed:affiliation |
Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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