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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2005-7-6
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pubmed:abstractText |
Virus-like particles containing the rotavirus (RV) internal proteins VP2 and VP6 (2/6-VLP) have been shown to induce serum and fecal antibodies as well as protection in mice after intranasal administration with a mutant of E. coli toxin, LT-R192G. To better understand the origin of fecal IgA induced by this protocol, we studied the RV-specific B cell response in systemic and mucosal lymphoid tissues using a flow cytometry assay that allows quantification and phenotypic characterization of RV-specific B lymphocytes. We also assessed the RV-specific antibody-secreting cells in the spleen and intestinal lamina propria (ILP). A remarkably high frequency of RV-specific B cells was found in the respiratory lymphoid tissues and spleen, of which only a minority expressed the alpha4beta7 integrin (intestinal homing receptor). In contrast, but in accordance with alpha4beta7 expression at the induction site, a very low response was observed in intestinal lymphoid tissues (mesenteric lymph nodes and ILP), which did not increase after a second immunization. Thus, intranasal immunization with a nonreplicating antigen does not induce an important number of RV-specific B cells with an intestinal homing profile.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin D,
http://linkedlifedata.com/resource/pubmed/chemical/VP2 protein, Rotavirus,
http://linkedlifedata.com/resource/pubmed/chemical/VP6 protein, Rotavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2122-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15948217-Administration, Intranasal,
pubmed-meshheading:15948217-Animals,
pubmed-meshheading:15948217-Antibodies,
pubmed-meshheading:15948217-Antigens, Viral,
pubmed-meshheading:15948217-B-Lymphocytes,
pubmed-meshheading:15948217-Bacterial Toxins,
pubmed-meshheading:15948217-Capsid Proteins,
pubmed-meshheading:15948217-Feces,
pubmed-meshheading:15948217-Immunoglobulin D,
pubmed-meshheading:15948217-Immunophenotyping,
pubmed-meshheading:15948217-Intestinal Mucosa,
pubmed-meshheading:15948217-Lymph Nodes,
pubmed-meshheading:15948217-Lymphocyte Activation,
pubmed-meshheading:15948217-Mice,
pubmed-meshheading:15948217-Mice, Inbred BALB C,
pubmed-meshheading:15948217-Mutation,
pubmed-meshheading:15948217-Nasal Mucosa,
pubmed-meshheading:15948217-Rotavirus,
pubmed-meshheading:15948217-Spleen,
pubmed-meshheading:15948217-Vaccines, Subunit,
pubmed-meshheading:15948217-Viral Proteins,
pubmed-meshheading:15948217-Viral Vaccines,
pubmed-meshheading:15948217-Virion
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pubmed:year |
2005
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pubmed:articleTitle |
Distribution and phenotype of murine rotavirus-specific B cells induced by intranasal immunization with 2/6 virus-like particles.
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pubmed:affiliation |
Microbiologie Médicale et Moléculaire, EA562, UFRs Médecine et Pharmacie, Laboratoire de Virologie CHU, Dijon, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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