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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5734
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pubmed:dateCreated |
2005-7-25
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pubmed:abstractText |
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD81,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CD81 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/NS-5 protein, hepatitis C virus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein E2, Hepatitis C virus
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1095-9203
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pubmed:author |
pubmed-author:BurtonDennis RDR,
pubmed-author:EvansMatthew JMJ,
pubmed-author:HynesRichard ORO,
pubmed-author:LindenbachBrett DBD,
pubmed-author:LiuChristopher CCC,
pubmed-author:MaruyamaToshiakiT,
pubmed-author:McKeatingJane AJA,
pubmed-author:RiceCharles MCM,
pubmed-author:SyderAndrew JAJ,
pubmed-author:TellinghuisenTimothy LTL,
pubmed-author:WölkBennoB
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pubmed:issnType |
Electronic
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pubmed:day |
22
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pubmed:volume |
309
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15947137-Antibodies, Monoclonal,
pubmed-meshheading:15947137-Antibodies, Viral,
pubmed-meshheading:15947137-Antigens, CD,
pubmed-meshheading:15947137-Antigens, CD81,
pubmed-meshheading:15947137-Antiviral Agents,
pubmed-meshheading:15947137-Cell Line, Tumor,
pubmed-meshheading:15947137-Centrifugation, Density Gradient,
pubmed-meshheading:15947137-Culture Media, Conditioned,
pubmed-meshheading:15947137-Genome, Viral,
pubmed-meshheading:15947137-Hepacivirus,
pubmed-meshheading:15947137-Humans,
pubmed-meshheading:15947137-Interferon-alpha,
pubmed-meshheading:15947137-Mutation,
pubmed-meshheading:15947137-Neutralization Tests,
pubmed-meshheading:15947137-RNA, Viral,
pubmed-meshheading:15947137-Replicon,
pubmed-meshheading:15947137-Serial Passage,
pubmed-meshheading:15947137-Transfection,
pubmed-meshheading:15947137-Viral Envelope Proteins,
pubmed-meshheading:15947137-Viral Nonstructural Proteins,
pubmed-meshheading:15947137-Virion,
pubmed-meshheading:15947137-Virus Cultivation,
pubmed-meshheading:15947137-Virus Replication
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pubmed:year |
2005
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pubmed:articleTitle |
Complete replication of hepatitis C virus in cell culture.
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pubmed:affiliation |
Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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