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rdf:type |
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lifeskim:mentions |
umls-concept:C0003695,
umls-concept:C0007134,
umls-concept:C0018270,
umls-concept:C0086418,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0599946,
umls-concept:C1289971,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C2917177
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pubmed:issue |
1
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pubmed:dateCreated |
2005-6-9
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pubmed:abstractText |
The metabolism of arachidonic acid by either the cyclooxygenase or lipoxygenase (LOX) pathway is believed to play an important role in tumor promotion. We investigated the expression of 5- and 12-LOX in renal cell carcinoma (RCC), as well as the effects of their inhibitors on cell proliferation in 2 RCC cell lines (Caki-1 and A498). Expression of 5- and 12-LOX was detected by immunohistochemistry and RT-PCR. Effects of LOX inhibitors on RCC cell growth were examined by MTT assay, and Hoechst staining was used to determine whether or not the LOX inhibitors induce apoptosis. While 5- and 12-LOX expression levels were slightly detected in NK tissues, marked expressions of 5- and 12-LOX were detected in RCC tissues. 5-LOX inhibitors caused marked reduction of RCC cells in a concentration- and time-dependent manner. The effect of the 5-LOX inhibitor was stronger than the 12-LOX inhibitor. Furthermore, the 5-LOX inhibitor caused a marked reduction of RCC cells through apoptosis. LOX, especially 5-LOX, is induced in RCC, and the results suggest that the 5-LOX inhibitor may mediate potent anti-proliferative effects against RCC cells. Thus, 5-LOX may become a new target in treatment of RCC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1021-335X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
73-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15944770-Aged,
pubmed-meshheading:15944770-Apoptosis,
pubmed-meshheading:15944770-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:15944770-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:15944770-Arachidonic Acid,
pubmed-meshheading:15944770-Carcinoma, Renal Cell,
pubmed-meshheading:15944770-Cell Line, Tumor,
pubmed-meshheading:15944770-Cell Proliferation,
pubmed-meshheading:15944770-Cell Survival,
pubmed-meshheading:15944770-Cells, Cultured,
pubmed-meshheading:15944770-Dose-Response Relationship, Drug,
pubmed-meshheading:15944770-Female,
pubmed-meshheading:15944770-Flavonoids,
pubmed-meshheading:15944770-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15944770-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15944770-Humans,
pubmed-meshheading:15944770-Immunohistochemistry,
pubmed-meshheading:15944770-Kidney,
pubmed-meshheading:15944770-Kidney Neoplasms,
pubmed-meshheading:15944770-Lipoxygenase Inhibitors,
pubmed-meshheading:15944770-Male,
pubmed-meshheading:15944770-Middle Aged,
pubmed-meshheading:15944770-Nordihydroguaiaretic Acid,
pubmed-meshheading:15944770-RNA, Messenger,
pubmed-meshheading:15944770-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15944770-Signal Transduction,
pubmed-meshheading:15944770-Time Factors
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pubmed:year |
2005
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pubmed:articleTitle |
5-Lipoxygenase inhibitors attenuate growth of human renal cell carcinoma and induce apoptosis through arachidonic acid pathway.
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pubmed:affiliation |
Department of Urology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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