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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-6-30
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pubmed:abstractText |
Transcripts derived from the 6 exon CALC I gene are differentially processed in a tissue-specific fashion to include or exclude a calcitonin-specific exon 4. All cell types which transcribe a second calcitonin/CGRP gene, CALC II, exclude exon 4. Substitution of the first 30 nucleotides of CALC I exon 4 with analogous CALC II sequence was sufficient to prevent recognition of exon 4 in in vitro or in vivo RNA splicing systems. UV crosslinking detected a approximately 66 kDa RNA-binding protein in HeLa nuclear extract which interacted with CALC I proximal exon sequence, but not CALC II or mutant sequences. UV crosslinking of this protein was inhibited by addition of nuclear extract from a cell type which normally causes exclusion of exon 4. These results identify an important regulatory element within exon 4 and support a model in which calcitonin production requires protein interaction with this sequence to facilitate exon recognition.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-1649451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-1674449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-1824871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-1902987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2026327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2216708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2280774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2316404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2427200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2427201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2505080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2524382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2639135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2788825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2798134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2832738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-2879637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3017190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3215513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3304142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3489641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3492393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-3493259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-6089345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-6283379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1594453-6828386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
20
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pubmed:geneSymbol |
CALC I
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2361-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1594453-Amino Acid Sequence,
pubmed-meshheading:1594453-Base Sequence,
pubmed-meshheading:1594453-Calcitonin,
pubmed-meshheading:1594453-Calcitonin Gene-Related Peptide,
pubmed-meshheading:1594453-DNA,
pubmed-meshheading:1594453-Exons,
pubmed-meshheading:1594453-HeLa Cells,
pubmed-meshheading:1594453-Humans,
pubmed-meshheading:1594453-Molecular Sequence Data,
pubmed-meshheading:1594453-Mutation,
pubmed-meshheading:1594453-Polymerase Chain Reaction,
pubmed-meshheading:1594453-RNA Splicing,
pubmed-meshheading:1594453-RNA-Binding Proteins,
pubmed-meshheading:1594453-Ultraviolet Rays
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pubmed:year |
1992
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pubmed:articleTitle |
Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP.
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pubmed:affiliation |
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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