Linked Life Data

15944003

Source:http://linkedlifedata.com/resource/pubmed/id/15944003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-6-9
pubmed:abstractText
Galanin, acting at the GalR1-3 subtypes of galanin receptors, is involved in the regulation of cognition, mood, feeding, seizure activity and pain. The understanding of galanin's effects in molecular and cellular terms has been hampered by the lack of receptor subtype selective ligands and antibodies. Previous in situ hybridization data showed that GalR1 and GalR2 receptors are abundant in the rat brain, while the distribution of GalR3 is contradictory and most studies demonstrated a low expression levels in the rat brain. The distribution of galanin receptor subtypes at protein level is unknown. In the present study, we report the regional distribution of the galanin receptors: GalR1 and non-GalR1 receptors, using a recently synthesized high affinity GalR2/3 selective ligand, galanin (2-11), and galanin (1-29), as competitors, in saturating (125)I-galanin membrane binding assay. We show that paraventricular nucleus (PVN) express predominantly GalR1, whereas areas like the dorsal raphe nucleus (DRN), hippocampus and amygdala express both the GalR1 and non-GalR1 receptors. We speculate that the GalR2/3 binding sites detected by galanin (2-11) binding in our study probably represent mostly GalR2 receptors. In addition, we show regionally specific and subtype specific regulation of galanin receptors. Status epilepticus (SE), known to deplete galanin from axonal projections of locus coeruleus and septum/diagonal band neurons in the hippocampus and to induce galanin expression in a subset of hippocampal cells, down regulates GalR2 receptor mRNA and proteins by 30% without altering the GalR1 receptors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0143-4179
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-52
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15944003-Amygdala, pubmed-meshheading:15944003-Animals, pubmed-meshheading:15944003-Brain, pubmed-meshheading:15944003-CHO Cells, pubmed-meshheading:15944003-Cricetinae, pubmed-meshheading:15944003-Down-Regulation, pubmed-meshheading:15944003-Galanin, pubmed-meshheading:15944003-Gene Expression, pubmed-meshheading:15944003-Hippocampus, pubmed-meshheading:15944003-Ligands, pubmed-meshheading:15944003-Male, pubmed-meshheading:15944003-Muscarinic Agonists, pubmed-meshheading:15944003-Paraventricular Hypothalamic Nucleus, pubmed-meshheading:15944003-Peptide Fragments, pubmed-meshheading:15944003-Pilocarpine, pubmed-meshheading:15944003-Raphe Nuclei, pubmed-meshheading:15944003-Rats, pubmed-meshheading:15944003-Rats, Sprague-Dawley, pubmed-meshheading:15944003-Receptor, Galanin, Type 1, pubmed-meshheading:15944003-Receptor, Galanin, Type 2, pubmed-meshheading:15944003-Receptor, Galanin, Type 3, pubmed-meshheading:15944003-Receptors, Galanin, pubmed-meshheading:15944003-Status Epilepticus
pubmed:year
2005
pubmed:articleTitle
Distribution and differential regulation of galanin receptor subtypes in rat brain: effects of seizure activity.
pubmed:affiliation
Department of Neuropharmacology, The Harold L. Dorris Neurological Research Center, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural