Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-9
pubmed:abstractText
Polymerase chain reaction (PCR) amplification using formalin-fixed material is very limited. In the present study the use of 6 week formalin-fixed placenta for individual identification was examined based on DNA analyses. The objective of the examination was to prove whether the placenta was from a woman who had just given birth. DNA extraction was carried out from the maternal blood sample and from the formalin-fixed placental samples composed of three parts: maternal side, infant side and umbilical cord. One minisatellite (D1S80), 12 short tandem repeat (STR) polymorphisms and amelogenin X, Y were investigated. All the polymorphic systems were detected in the maternal blood sample. The majority of the DNA isolated from the placental tissues had molecular weights of approximately 500 bp, and only two to four STR loci were amplified using the DNA. In order to amplify more DNA polymorphic markers from the formalin-fixed tissues, whole genome amplification was performed. After amplification by degenerate oligonucleotide-primed PCR (DOP-PCR), the products contained DNA with increased molecular weight up to >10 kbp. More DNA loci were typed using the DOP-PCR products. Furthermore, large molecular size fragments were purified from the DOP-PCR products by agarose electrophoresis, and then the D1S80 locus and 12 STR loci were successfully amplified using these fragments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1320-5463
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15943791-Adult, pubmed-meshheading:15943791-Amelogenin, pubmed-meshheading:15943791-DNA, pubmed-meshheading:15943791-Dental Enamel Proteins, pubmed-meshheading:15943791-Female, pubmed-meshheading:15943791-Forensic Medicine, pubmed-meshheading:15943791-Formaldehyde, pubmed-meshheading:15943791-Genetic Markers, pubmed-meshheading:15943791-Genome, Human, pubmed-meshheading:15943791-Humans, pubmed-meshheading:15943791-Infant, pubmed-meshheading:15943791-Male, pubmed-meshheading:15943791-Microsatellite Repeats, pubmed-meshheading:15943791-Placenta, pubmed-meshheading:15943791-Polymerase Chain Reaction, pubmed-meshheading:15943791-Polymorphism, Genetic, pubmed-meshheading:15943791-Pregnancy, pubmed-meshheading:15943791-Tissue Fixation, pubmed-meshheading:15943791-Umbilical Cord
pubmed:year
2005
pubmed:articleTitle
Individual identification by DNA polymorphism using formalin-fixed placenta with whole genome amplification.
pubmed:affiliation
Department of Legal Medicine, Nihon University School of Medicine, Oyaguchi Kamimachi, Itabashi, Japan. jtie@med.nihon-u.ac.jp
pubmed:publicationType
Journal Article, Case Reports