Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-9
pubmed:abstractText
Three cell systems (MINT1/2/3) derived from a melanotic neuroectodermal tumor of infancy (MNTI) arising in the mandible of a 1-month-old newborn boy have been established, and their cytological natures have been characterized. The cells had immunopositivities for pan-keratin, vimentin, neuron-specific enolase, S-100 protein and melanoma-associated antigen (HMB-45). These immunohistochemical phenotypes were basically the same as those observed in tissue sections, in which, synaptophysin, myelin basic protein, c-myc gene products, carcinoembryonic antigen, and epithelial membrane antigen were also immunolocalized in tumor cells. Karyotyping analyzes revealed that the chromosome numbers of the three cell systems ranged from 60 to 67 with 3n ploidies, and that there were many structural aberrations, such as del(11)(q13), del(22)(q13), add(2)(p11), add(7)(q22), extra copies for chromosomes 1, 2, 3, 5, 7, 9, 10, 11, 12, 16, 20, and 22, der(9)t(9;13)(p13;q12)add(9)(q34), and der(13;21)(q10;q10), which were shared by the three cell systems, while der(19)t(11;19)(q13;p13) was found in MINT1 and MINT3. When stimulated by endothelin-3 and vitamin D(3), the cells had spinous cell shapes with immunopositivities for HMB-45, neurofilament protein and glial fibrillary acidic protein, which indicated more neural differentiation. The established cell systems will be useful for further investigation on the molecular and genetic basis of MNTI to understand its pathogenesis, which is largely unknown.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Carcinoembryonic Antigen, http://linkedlifedata.com/resource/pubmed/chemical/Cholecalciferol, http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-3, http://linkedlifedata.com/resource/pubmed/chemical/Keratins, http://linkedlifedata.com/resource/pubmed/chemical/MYC protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Melanoma-Specific Antigens, http://linkedlifedata.com/resource/pubmed/chemical/Mucin-1, http://linkedlifedata.com/resource/pubmed/chemical/Myelin Basic Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc, http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Vimentin
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1320-5463
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-42
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15943790-Animals, pubmed-meshheading:15943790-Antigens, Neoplasm, pubmed-meshheading:15943790-Carcinoembryonic Antigen, pubmed-meshheading:15943790-Cell Differentiation, pubmed-meshheading:15943790-Cell Line, Tumor, pubmed-meshheading:15943790-Cholecalciferol, pubmed-meshheading:15943790-Chromosome Aberrations, pubmed-meshheading:15943790-Endothelin-3, pubmed-meshheading:15943790-Female, pubmed-meshheading:15943790-Fluorescent Antibody Technique, pubmed-meshheading:15943790-Humans, pubmed-meshheading:15943790-Immunohistochemistry, pubmed-meshheading:15943790-Infant, Newborn, pubmed-meshheading:15943790-Karyotyping, pubmed-meshheading:15943790-Keratins, pubmed-meshheading:15943790-Male, pubmed-meshheading:15943790-Mandibular Neoplasms, pubmed-meshheading:15943790-Melanoma-Specific Antigens, pubmed-meshheading:15943790-Mice, pubmed-meshheading:15943790-Mice, Inbred BALB C, pubmed-meshheading:15943790-Mice, Nude, pubmed-meshheading:15943790-Mucin-1, pubmed-meshheading:15943790-Myelin Basic Proteins, pubmed-meshheading:15943790-Neoplasm Proteins, pubmed-meshheading:15943790-Neoplasm Transplantation, pubmed-meshheading:15943790-Neoplasms, Experimental, pubmed-meshheading:15943790-Neuroectodermal Tumor, Melanotic, pubmed-meshheading:15943790-Phosphopyruvate Hydratase, pubmed-meshheading:15943790-Proto-Oncogene Proteins c-myc, pubmed-meshheading:15943790-S100 Proteins, pubmed-meshheading:15943790-Transplantation, Heterologous, pubmed-meshheading:15943790-Vimentin
pubmed:year
2005
pubmed:articleTitle
Establishment and characterization of new cell lines derived from melanotic neuroectodermal tumor of infancy arising in the mandible.
pubmed:affiliation
Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't