Linked Life Data

15940506

Source:http://linkedlifedata.com/resource/pubmed/id/15940506

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-7-15
pubmed:abstractText
To examine the stability of bone matrix proteins for crystal dislocation, the immunolocalization of type I collagen, bone sialoprotein, and osteopontin was investigated during different stages of fixation and decalcification. Four-week-old rat femurs were rapidly frozen, and were sectioned without fixation or decalcification. Thereafter, following or bypassing fixation in 4% paraformaldehyde, these sections were decalcified in 5% EDTA for 0-5 min. Before decalcification, marked radiopacity of bone matrix was observed in contact microradiography (CMR) images, and electron probe microanalysis (EPMA) demonstrated intense localization for phosphorus and calcium. In fixed and unfixed sections without decalcification, immunolocalization of bone matrix proteins were almost restricted to osteoid. After 1 min of decalcification, reduced radiopacity was apparent in the CMR images, and less phosphorus and calcium was observed by EPMA, which completely disappeared by 5 min decalcification. After 3-5 min of decalcification, unfixed sections showed that these proteins were immunolocalized in bone matrix, but were not detectable in osteoid. However, fixed sections demonstrated that these were found in both bone matrix and osteoid. The present findings suggest that bone matrix proteins are embedded in calcified matrix which is separated from the aqueous environment and that they hardly move, probably due to firm bonding with each other. In contrast, matrix proteins in osteoid are subject to loss after decalcification because they may be bound to scattered apatite crystals, not to each other.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0948-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
639-46
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15940506-Animals, pubmed-meshheading:15940506-Bone Matrix, pubmed-meshheading:15940506-Collagen Type I, pubmed-meshheading:15940506-Decalcification Technique, pubmed-meshheading:15940506-Edetic Acid, pubmed-meshheading:15940506-Electron Probe Microanalysis, pubmed-meshheading:15940506-Femur, pubmed-meshheading:15940506-Formaldehyde, pubmed-meshheading:15940506-Frozen Sections, pubmed-meshheading:15940506-Immunohistochemistry, pubmed-meshheading:15940506-Integrin-Binding Sialoprotein, pubmed-meshheading:15940506-Microradiography, pubmed-meshheading:15940506-Osteopontin, pubmed-meshheading:15940506-Polymers, pubmed-meshheading:15940506-Rats, pubmed-meshheading:15940506-Rats, Wistar, pubmed-meshheading:15940506-Sialoglycoproteins, pubmed-meshheading:15940506-Staining and Labeling, pubmed-meshheading:15940506-Tissue Fixation
pubmed:year
2005
pubmed:articleTitle
Effects of fixation and decalcification on the immunohistochemical localization of bone matrix proteins in fresh-frozen bone sections.
pubmed:affiliation
Department of Oral Histology, Matsumoto Dental University, 1780 Gobara Hirooka, Shiojiri, Nagano, 399-0781, Japan. hosoya@po.mdu.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't