Source:http://linkedlifedata.com/resource/pubmed/id/15937965
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2005-6-8
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pubmed:abstractText |
Proteome analysis of Gram-negative facultative intracellular pathogen Francisella tularensis (F. tularensis) live vaccine strain has been performed only on whole-cell extracts so far. This is the first study dealing with the analysis of the membrane subproteome of this microorganism. A fraction enriched in membrane proteins obtained by carbonate extraction was separated using two-dimensional electrophoresis and all visualized spots were identified by mass spectrometry. The reference map is the basis for further comparative analyses of virulent and non-virulent F. tularensis strains.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1615-9853
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2460-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15937965-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:15937965-Francisella tularensis,
pubmed-meshheading:15937965-Membrane Proteins,
pubmed-meshheading:15937965-Proteomics,
pubmed-meshheading:15937965-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:15937965-Vaccines, Attenuated
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pubmed:year |
2005
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pubmed:articleTitle |
Francisella tularensis live vaccine strain: proteomic analysis of membrane proteins enriched fraction.
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pubmed:affiliation |
Proteome Center for the Study of Intracellular Parasitism of Bacteria, Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic. klimtova@pmfhk.cz
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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