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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2005-8-15
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pubmed:abstractText |
Recent studies have indicated that mast cells play an intermediate role in chemokine-induced neutrophil recruitment in vivo. The aim of the present investigation was to determine the role of tumour necrosis factor-alpha (TNF-alpha) in neutrophil recruitment provoked by the CXC chemokine macrophage inflammatory protein-2 (MIP-2). For this purpose, we used mast cell- and TNF-alpha-deficient mice and studied neutrophil adhesion to endothelial cells in vitro and neutrophil recruitment in the mouse cremaster muscle in vivo. In contrast to the classical chemoattractant formyl-methionine-leucine-phenylalanin (fMLP), MIP-2 dose dependently increased neutrophil accumulation in vivo. This MIP-2-regulated neutrophil recruitment was abolished in mast cell-deficient mice. TNF-alpha increased E-selectin mRNA expression in both wild-type (WT) and mast cell-deficient mice. In contrast, MIP-2 challenge increased gene expression of E-selectin in WT but not in mast cell-deficient animals. Moreover, MIP-2-provoked extravascular accumulation of neutrophils was reduced by 78% in mice lacking TNF-alpha. In order to better define the role of mast cell-derived TNF-alpha in neutrophil responses to MIP-2, we used an in vitro endothelial cell adhesion assay with and without mast cells. Interestingly, MIP-2-induced neutrophil adhesion to endothelial cells was decreased by 58% using TNF-alpha-deficient compared to WT mast cells. Moreover, mast cell secretion of TNF-alpha increased by more than 71% in response to challenge with MIP-2. Taken together, our results suggest that MIP-2-induced neutrophil recruitment is mediated by TNF-alpha released from local mast cells. These findings help to explain the complex molecular interactions between chemokines, mast cell activation and neutrophil infiltration in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-10216072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-10477904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-10479407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-10570305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-10961383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-11085746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-11375258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-11399672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-11935287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-12454516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-12488493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-12598424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-1378846,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-1718038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-1760836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-2201751,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-2917992,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15937521-9987599
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1062-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15937521-Animals,
pubmed-meshheading:15937521-Cell Adhesion,
pubmed-meshheading:15937521-Chemokine CXCL2,
pubmed-meshheading:15937521-Chemokines,
pubmed-meshheading:15937521-Dose-Response Relationship, Drug,
pubmed-meshheading:15937521-E-Selectin,
pubmed-meshheading:15937521-Endothelial Cells,
pubmed-meshheading:15937521-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15937521-Male,
pubmed-meshheading:15937521-Mast Cells,
pubmed-meshheading:15937521-Mice,
pubmed-meshheading:15937521-Mice, Inbred Strains,
pubmed-meshheading:15937521-Neutrophil Infiltration,
pubmed-meshheading:15937521-Neutrophils,
pubmed-meshheading:15937521-RNA, Messenger,
pubmed-meshheading:15937521-Receptors, Interleukin-8B,
pubmed-meshheading:15937521-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15937521-Tumor Necrosis Factor-alpha
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pubmed:year |
2005
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pubmed:articleTitle |
Mast cell-derived tumour necrosis factor-alpha mediates macrophage inflammatory protein-2-induced recruitment of neutrophils in mice.
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pubmed:affiliation |
Department of Surgery, Malmö University Hospital, Lund University, S-205 02 Malmö, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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