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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-6-6
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pubmed:abstractText |
The kinase PAR-1 plays conserved roles in cell polarity. PAR-1 has also been implicated in axis establishment in C. elegans and Drosophila and in Wnt signaling, but its role in vertebrate development is unclear. Here we report that PAR-1 has two distinct and essential roles in axial development in Xenopus mediated by different PAR-1 isoforms. Depletion of PAR-1A or PAR-1BX causes dorsoanterior deficits, reduced Spemann organizer gene expression, and inhibition of canonical Wnt-beta-catenin signaling. By contrast, PAR-1BY depletion inhibits cell movements and localization of Dishevelled protein to the cell cortex, processes associated with noncanonical Wnt signaling. PAR-1 phosphorylation sites in Dishevelled are required for this translocation, but not for canonical Wnt signaling. We conclude that PAR-1BY is required in the PCP branch and mediates Dsh membrane localization while PAR-1A and PAR-1BX are essential for canonical signaling to beta-catenin, possibly via targets other than Dishevelled.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Par-1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/beta-catenin protein, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1534-5807
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
829-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15935773-Animals,
pubmed-meshheading:15935773-Animals, Genetically Modified,
pubmed-meshheading:15935773-Autoradiography,
pubmed-meshheading:15935773-Blotting, Western,
pubmed-meshheading:15935773-Body Patterning,
pubmed-meshheading:15935773-Cell Membrane,
pubmed-meshheading:15935773-Cytoskeletal Proteins,
pubmed-meshheading:15935773-Dose-Response Relationship, Drug,
pubmed-meshheading:15935773-Enzyme Activation,
pubmed-meshheading:15935773-Gene Expression Regulation, Developmental,
pubmed-meshheading:15935773-Green Fluorescent Proteins,
pubmed-meshheading:15935773-In Situ Hybridization,
pubmed-meshheading:15935773-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:15935773-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:15935773-Luciferases,
pubmed-meshheading:15935773-Microinjections,
pubmed-meshheading:15935773-Oligonucleotides, Antisense,
pubmed-meshheading:15935773-Phenotype,
pubmed-meshheading:15935773-Phosphorylation,
pubmed-meshheading:15935773-Protein Biosynthesis,
pubmed-meshheading:15935773-Protein Isoforms,
pubmed-meshheading:15935773-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15935773-RNA,
pubmed-meshheading:15935773-Signal Transduction,
pubmed-meshheading:15935773-Tissue Culture Techniques,
pubmed-meshheading:15935773-Trans-Activators,
pubmed-meshheading:15935773-Wnt Proteins,
pubmed-meshheading:15935773-Xenopus,
pubmed-meshheading:15935773-Xenopus Proteins,
pubmed-meshheading:15935773-Xenopus laevis,
pubmed-meshheading:15935773-beta Catenin
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pubmed:year |
2005
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pubmed:articleTitle |
Distinct PAR-1 proteins function in different branches of Wnt signaling during vertebrate development.
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pubmed:affiliation |
Department of Cancer Biology, Dana Farber Cancer Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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