Linked Life Data

15935378

Source:http://linkedlifedata.com/resource/pubmed/id/15935378

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-6-14
pubmed:abstractText
Mitochondrial NADH dehydrogenase (complex I) of plants includes quite a number of plant-specific subunits, some of which exhibit sequence similarity to bacterial gamma-carbonic anhydrases. A homozygous Arabidopsis knockout mutant carrying a T-DNA insertion in a gene encoding one of these subunits (At1g47260) was generated to investigate its physiological role. Isolation of mitochondria and separation of mitochondrial protein complexes by Blue-native polyacrylamide gel electrophoresis or sucrose gradient ultracentrifugation revealed drastically reduced complex I levels. Furthermore, the mitochondrial I + III2 supercomplex was very much reduced in mutant plants. Remaining complex I had normal molecular mass, suggesting substitution of the At1g47260 protein by one or several of the structurally related subunits of this respiratory protein complex. Immune-blotting experiments using polyclonal antibodies directed against the At1g47260 protein indicated its presence within complex I, the I + III2 supercomplex and smaller protein complexes, which possibly represent subcomplexes of complex I. Changes within the mitochondrial proteome of mutant cells were systematically monitored by fluorescence difference gel electrophoresis using 2D Blue-native/SDS and 2D isoelectric focussing/SDS polyacrylamide gel electrophoresis. Complex I subunits are largely absent within the mitochondrial proteome. Further mitochondrial proteins are reduced in mutant plants, like mitochondrial ferredoxin, others are increased, like formate dehydrogenase. Development of mutant plants was normal under standard growth conditions. However, a suspension cell culture generated from mutant plants exhibited clearly reduced growth rates and respiration. In summary, At1g47260 is important for complex I assembly in plant mitochondria and respiration. A role of At1g47260 in mitochondrial one-carbon metabolism is supported by micro-array analyses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
350
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15935378-Arabidopsis, pubmed-meshheading:15935378-Carbon, pubmed-meshheading:15935378-Carbonic Anhydrases, pubmed-meshheading:15935378-Cell Nucleus, pubmed-meshheading:15935378-Cell Respiration, pubmed-meshheading:15935378-Cells, Cultured, pubmed-meshheading:15935378-Centrifugation, Density Gradient, pubmed-meshheading:15935378-DNA, Bacterial, pubmed-meshheading:15935378-Electron Transport Complex I, pubmed-meshheading:15935378-Electron Transport Complex III, pubmed-meshheading:15935378-Gene Deletion, pubmed-meshheading:15935378-Mitochondria, pubmed-meshheading:15935378-Mitochondrial Proteins, pubmed-meshheading:15935378-Mutagenesis, Insertional, pubmed-meshheading:15935378-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15935378-Phenotype, pubmed-meshheading:15935378-Protein Transport, pubmed-meshheading:15935378-Proteome
pubmed:year
2005
pubmed:articleTitle
Disruption of a nuclear gene encoding a mitochondrial gamma carbonic anhydrase reduces complex I and supercomplex I + III2 levels and alters mitochondrial physiology in Arabidopsis.
pubmed:affiliation
Institut für Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't