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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-7-2
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pubmed:abstractText |
1. The lungs of five fetal (133-140 days gestation) and thirty-four postnatal (2-240 days) sheep were artificially perfused in situ with warmed and oxygenated sheep blood. In postnatal animals the airspace of the lung was filled with liquid similar in composition to fetal lung liquid. In fetal and postnatal animals luminal liquid volume was measured by the impermeant tracer technique. 2. Under resting conditions the pulmonary epithelium of fetal animals secreted liquid at a mean (+/- S.E.M.) rate of 2.0 (+/- 0.4) ml (kg body weight)-1 h-1, those of postnantal animals absorbed liquid at -1.8 (+/- 0.2) ml (kg body weight)-1 h-1. 3. Addition of 2,4-dinitrophenol to achieve a concentration of 1.5 x 10(-3) M in the perfusing blood in postnatal animals caused complete cessation of liquid absorption. 4. Light and electron microscopic examination of the lung after periods of up to 6 h of artificial perfusion showed no evidence of epithelial damage. From 3 h onwards, liquid accumulation was evident in the perivascular spaces. 5. Addition of adrenaline to the perfusate in fetal animals caused absorption of liquid to occur at a mean rate of -2.9 (+/- 1.3) ml (kg body weight)-1 h-1. In postnatal animals adrenaline caused the rate of liquid absorption to increase from a mean rate of -1.4 (+/- 0.2) to -2.2 (+/- 0.3) ml (kg body weight)-1 h-1. 6. In the fetus addition of amiloride (0.8 x 10(-4) M) to the luminal fluid blocked adrenaline-induced liquid absorption and caused secretion to occur at 1.3 (+/- 0.3) ml (kg body weight)-1 h-1. 7. In postnatal animals the response to amiloride was age dependent. In newborn lambs (2-14 days) amiloride blocked liquid absorption and caused secretion of liquid to occur in seven out of eight animals at a mean rate of 0.9 (+/- 0.3) ml (kg body weight)-1 h-1 (n = 8). In older animals (15-240 days) the characteristic response to amiloride was slowing of the rate of liquid absorption (mean rate of absorption,-0.2 (+/- 0.09) ml (kg body weight)-1 h-1, n = 18) with liquid secretion being seen in only three of eighteen animals.(ABSTRACT TRUNCATED AT 400 WORDS)
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-16991717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-1841951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-205826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-2585288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-2842724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3109762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3116209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3382652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3393394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3575905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3656149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3795077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-3944038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-4055578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-4443921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-5158386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-5171649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-517666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-6149212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-6655575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-6964398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-7118651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-7310716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1593479-7399999
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
448
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
579-97
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:1593479-2,4-Dinitrophenol,
pubmed-meshheading:1593479-Absorption,
pubmed-meshheading:1593479-Aging,
pubmed-meshheading:1593479-Amiloride,
pubmed-meshheading:1593479-Animals,
pubmed-meshheading:1593479-Dinitrophenols,
pubmed-meshheading:1593479-Epinephrine,
pubmed-meshheading:1593479-Epithelium,
pubmed-meshheading:1593479-Extravascular Lung Water,
pubmed-meshheading:1593479-Fetus,
pubmed-meshheading:1593479-Lung,
pubmed-meshheading:1593479-Oxygen Consumption,
pubmed-meshheading:1593479-Secretory Rate,
pubmed-meshheading:1593479-Sheep
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pubmed:year |
1992
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pubmed:articleTitle |
Liquid flow across the epithelium of the artificially perfused lung of fetal and postnatal sheep.
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pubmed:affiliation |
Department of Paediatrics, University College London.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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