Source:http://linkedlifedata.com/resource/pubmed/id/15927395
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-7-11
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| pubmed:abstractText |
The neuropeptide calcitonin gene-related peptide (CGRP) binds to a subpopulation of dorsal root ganglion (DRG) neurons, elevates intracellular calcium, and causes inward currents in about 30% of lumbar DRG neurons. Using whole-cell patch clamp recordings, we found in the present study that application of CGRP to isolated and cultured DRG neurons from the adult rat enhances voltage-gated TTX-resistant (TTX-R) Na(+) inward currents in about 30% of small- to medium-sized DRG neurons. During CGRP, peak densities of Na(+) currents increased significantly. CGRP shifted the membrane conductance of the CGRP-responsive cells towards hyperpolarization without changing the slope of the peak conductance curve. The effect of CGRP was blocked by coadministration of CGRP8-37, an antagonist at the CGRP receptor. The effect of CGRP was also blocked after bath application of PKA14-22, a membrane-permeant blocker of protein kinase A, and PKC19-31, a PKC inhibitor, in the recording pipette. These data show pronounced facilitatory effects of CGRP on TTX-R Na(+) currents in DRG neurons which are mediated through CGRP receptors and intracellular pathways involving protein kinases A and C. Thus, in addition to prostaglandins, CGRP is another mediator that affects TTX-R Na(+) currents which are thought to occur mainly in nociceptive DRG neurons.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Local,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/protein kinase C (19-31)
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0304-3959
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
116
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
194-204
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15927395-Action Potentials,
pubmed-meshheading:15927395-Anesthetics, Local,
pubmed-meshheading:15927395-Animals,
pubmed-meshheading:15927395-Calcitonin Gene-Related Peptide,
pubmed-meshheading:15927395-Cells, Cultured,
pubmed-meshheading:15927395-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:15927395-Dose-Response Relationship, Radiation,
pubmed-meshheading:15927395-Drug Interactions,
pubmed-meshheading:15927395-Electric Stimulation,
pubmed-meshheading:15927395-Ganglia, Spinal,
pubmed-meshheading:15927395-Male,
pubmed-meshheading:15927395-Neurons,
pubmed-meshheading:15927395-Patch-Clamp Techniques,
pubmed-meshheading:15927395-Peptide Fragments,
pubmed-meshheading:15927395-Protein Kinase C,
pubmed-meshheading:15927395-Rats,
pubmed-meshheading:15927395-Rats, Wistar,
pubmed-meshheading:15927395-Sodium Channels,
pubmed-meshheading:15927395-Tetrodotoxin,
pubmed-meshheading:15927395-Time Factors
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| pubmed:year |
2005
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| pubmed:articleTitle |
Calcitonin gene-related peptide enhances TTX-resistant sodium currents in cultured dorsal root ganglion neurons from adult rats.
|
| pubmed:affiliation |
Institut für Physiologie, University of Jena, Teichgraben 8, D-07740 Jena, Germany.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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