Linked Life Data

1592686

Source:http://linkedlifedata.com/resource/pubmed/id/1592686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-7-2
pubmed:abstractText
Transfer of imipenem resistance in Bacteroides fragilis was studied. Clinical isolate B. fragilis 10-73 was highly resistant to imipenem. Imipenem resistance was transferred from 10-73 to B. fragilis strain TM4000 at a frequency of 10(-6)/input recipient by a filter mating technique. The resistance could also be retransferred. B. fragilis 10-73 and both primary and secondary transcipients produced an imipenem-hydrolyzing metallo-beta-lactamase. Acquisition of imipenem resistance correlated with the appearance of plasmid DNA with a size (ca. 13.6 kb) similar to that of the donor strain. TM4000 transformed by electroporation with purified DNA of the 13.6-kb plasmid pBFUK1 produced the metallo-beta-lactamase and was resistant to imipenem. Transfer was resistant to DNase treatment and no transfer was seen with a sterile filtrate of the donor culture. It is suggested that gene transfer in B. fragilis has the properties of a conjugation system rather than those of transformation or transduction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-8820
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
542-7
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Conjugal transfer of imipenem resistance in Bacteroides fragilis.
pubmed:affiliation
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Japan.
pubmed:publicationType
Journal Article