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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0007589,
umls-concept:C0018120,
umls-concept:C0018270,
umls-concept:C0020885,
umls-concept:C0031272,
umls-concept:C0036945,
umls-concept:C0205409,
umls-concept:C0221971,
umls-concept:C0936012,
umls-concept:C1511938,
umls-concept:C1571705
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pubmed:issue |
4
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pubmed:dateCreated |
1992-7-2
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pubmed:abstractText |
We developed a method to isolate and culture cells from the lymphoid follicles of the ileal Peyer's (PP) patch of young sheep (6-12 weeks). These cells were 98% sIgM+ B cells and 1% T cells. Cultured follicular cells were used to investigate B-cell proliferation and differentiation. Less than 50% of B cells were viable after 24 hr of culture and this decrease in B-cell viability also occurred following co-stimulation with pokeweed mitogen (PWM) and recombinant bovine interleukin-1 (rBoIL-1) or rBoIL-2. In contrast, co-stimulation with PWM and either rBoIL-1 or rBoIL-2 induced a marked proliferative response that was maximal on Day 4 of culture. Cytokine-induced proliferation of the B cells required PWM co-stimulation and proliferation induced by rBoIL-1 and rBoIL-2 was neither additive or synergistic. This suggests that PWM bound a molecule or molecules that signalled responsiveness to both rBoIL-1 and rBoIL-2. Culture of follicular cells with PWM and both rBoIL-1 and rBoIL-2 also resulted in B-cell differentiation. This differentiation was associated with decreased proliferation, an increased number of viable B cells, and increased expression of both surface IgM and non-Ig membrane molecules. Thus, co-stimulation of ileal PP follicular cells with PWM and rBoIL-1 and rBoIL-2 resulted in both B-cell proliferation and differentiation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-1696040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-1900459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-2147525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-2431042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-2866070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3112218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3261832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3324464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3484719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3509948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3569406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3767761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3877937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3894225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-3950407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6222114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6333388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6334854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6420166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6434689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6602200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-6884422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592435-794413
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
601-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1592435-Animals,
pubmed-meshheading:1592435-B-Lymphocytes,
pubmed-meshheading:1592435-Cell Division,
pubmed-meshheading:1592435-Cell Survival,
pubmed-meshheading:1592435-Cells, Cultured,
pubmed-meshheading:1592435-Ileum,
pubmed-meshheading:1592435-Interleukin-1,
pubmed-meshheading:1592435-Interleukin-2,
pubmed-meshheading:1592435-Lymphocyte Subsets,
pubmed-meshheading:1592435-Peyer's Patches,
pubmed-meshheading:1592435-Pokeweed Mitogens,
pubmed-meshheading:1592435-Sheep
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pubmed:year |
1992
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pubmed:articleTitle |
An analysis of the growth and differentiation of B cells isolated from follicles of the ileal Peyer's patch of sheep.
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pubmed:affiliation |
Department of Medical Physiology, University of Calgary, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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