Linked Life Data

15923673

Source:http://linkedlifedata.com/resource/pubmed/id/15923673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2005-5-30
pubmed:abstractText
The quantum dot is a novel fluorescent platform that has the potential to become an alternative to conventional organic dyes used to label biological probes such as antibodies or ligands. Compared to typical fluorescent organic dyes, cadmium selenide/zinc sulfide core-shell nanocrystals, or quantum dots, have greater photostability, resist metabolic and chemical degradation, are nontoxic, and display broad emission and narrow excitation bands. When conjugated to generic adaptor molecules such as streptavidin, quantum dots can be used to label different biotinylated antibodies or ligands without having to customize the quantum dot surface chemistry for each antibody or ligand. In this chapter, we outline the methodology for using streptavidin quantum dots to label biotinylated antibodies that target cell-surface ectodomain proteins on both living and fixed cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
303
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-50
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Labeling cell-surface proteins via antibody quantum dot streptavidin conjugates.
pubmed:affiliation
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN, USA.
pubmed:publicationType
Journal Article