Linked Life Data

15923650

Source:http://linkedlifedata.com/resource/pubmed/id/15923650

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 11
pubmed:dateCreated
2005-5-30
pubmed:abstractText
Tumor necrosis factor alpha-converting enzyme (TACE/ADAM17) has been implicated in the inducible shedding of numerous cell surface proteins. In light of this, the regulation of TACE catalytic activation and protein maturation has recently received considerable interest in the context of extracellular signal-regulated kinase activation and the subsequent phosphorylation of TACE at residue Thr735. In this study, we analysed the subcellular localisation of TACE derivatives using laser-scanning confocal microscopy and cell surface biotinylation. Whereas WT.TACE- and T735A.TACE-enhanced green fluorescent protein (-eGFP) fusion derivatives of TACE were both found to localise with the endoplasmic reticulum, a phosphomimicking form of TACE (T735E.TACE-eGFP) was found to colocalise predominantly with components of the protein secretory pathway (COPII vesicles and trans-Golgi network). Additionally, upon analysis of biotinylated ectopic TACE, we observed that inducible trafficking of TACE to the cell surface was dependent upon extracellular signal-regulated kinase activation and phosphorylation of Thr735. We conclude from our observations that phosphorylation of TACE at Thr735 highlights a key step in inducible TACE protein trafficking and maturation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2371-80
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15923650-ADAM Proteins, pubmed-meshheading:15923650-Amino Acid Substitution, pubmed-meshheading:15923650-Animals, pubmed-meshheading:15923650-COP-Coated Vesicles, pubmed-meshheading:15923650-COS Cells, pubmed-meshheading:15923650-Cercopithecus aethiops, pubmed-meshheading:15923650-Endoplasmic Reticulum, pubmed-meshheading:15923650-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:15923650-Golgi Apparatus, pubmed-meshheading:15923650-HeLa Cells, pubmed-meshheading:15923650-Humans, pubmed-meshheading:15923650-Metalloendopeptidases, pubmed-meshheading:15923650-Phosphorylation, pubmed-meshheading:15923650-Point Mutation, pubmed-meshheading:15923650-Protein Processing, Post-Translational, pubmed-meshheading:15923650-Protein Transport, pubmed-meshheading:15923650-Threonine
pubmed:year
2005
pubmed:articleTitle
ERK-mediated phosphorylation of Thr735 in TNFalpha-converting enzyme and its potential role in TACE protein trafficking.
pubmed:affiliation
University of Cambridge, Department of Oncology, Cambridge Institute of Medical Research, Hills Road, Cambridge, CB2 2XY, UK. sms45@cam.ac.uk
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural