Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-10-3
pubmed:abstractText
In this study, we demonstrated that bcl-2 overexpression in human melanoma cells consistently enhanced the activity of multiple metastasis-related proteinases, in vitro cell invasion, and in vivo tumor growth. In particular, by using the M14 parental cell line, the MN8 control clone, and two bcl-2 overexpressing derivatives, we found that bcl-2 overexpressing cells exposed to hypoxia, when compared to parental cells, expressed higher level of several metalloproteases (MMPs) such as MMP-2, MMP-7, MT1-MMP, and tissue inhibitors of metalloproteases-1 and -2. Moreover, bcl-2 overexpression in melanoma cells enhanced in vitro invasion on matrigel and, in vivo tumor growth. The more aggressive behavior of bcl-2 transfectants tumors is significantly associated to an increase in MMP-2 expression as well as in a more elevated microvessel density as compared to the parental line. Taken together, our data suggest that bcl-2 plays a pivotal role in the regulation of molecules associated with the migratory and invasive phenotype, contributing, in cooperation to hypoxia, to tumor progression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
(c) 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
205
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
414-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15920759-Animals, pubmed-meshheading:15920759-Apoptosis, pubmed-meshheading:15920759-Biocompatible Materials, pubmed-meshheading:15920759-Cell Division, pubmed-meshheading:15920759-Cell Hypoxia, pubmed-meshheading:15920759-Cell Line, Tumor, pubmed-meshheading:15920759-Collagen, pubmed-meshheading:15920759-Disease Progression, pubmed-meshheading:15920759-Drug Combinations, pubmed-meshheading:15920759-Female, pubmed-meshheading:15920759-Humans, pubmed-meshheading:15920759-Immunohistochemistry, pubmed-meshheading:15920759-Integrins, pubmed-meshheading:15920759-Laminin, pubmed-meshheading:15920759-Matrix Metalloproteinases, pubmed-meshheading:15920759-Melanoma, pubmed-meshheading:15920759-Mice, pubmed-meshheading:15920759-Mice, Nude, pubmed-meshheading:15920759-Neoplasm Invasiveness, pubmed-meshheading:15920759-Neoplasm Transplantation, pubmed-meshheading:15920759-Proteoglycans, pubmed-meshheading:15920759-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:15920759-Tissue Inhibitor of Metalloproteinases, pubmed-meshheading:15920759-Transplantation, Heterologous
pubmed:year
2005
pubmed:articleTitle
Bcl-2 overexpression in melanoma cells increases tumor progression-associated properties and in vivo tumor growth.
pubmed:affiliation
Experimental Chemotherapy Laboratory, Regina Elena Cancer Institute, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't