|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
2005-5-27
|
|
pubmed:abstractText |
RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena of RNA interference in animals and posttranscriptional gene silencing (PTGS) in plants. In plants, PTGS acts as an antiviral system; a successful virus infection requires suppression or evasion of the induced silencing response. Small interfering RNAs (siRNAs) accumulate in plants infected with positive-strand RNA viruses and provide specificity to this RNA-mediated defense. We present here the results of a survey of virus-specific siRNAs characterized by a sequence analysis of siRNAs from plants infected with Cymbidium ringspot tombusvirus (CymRSV). CymRSV siRNA sequences have a nonrandom distribution along the length of the viral genome, suggesting that there are hot spots for virus-derived siRNA generation. CymRSV siRNAs bound to the CymRSV p19 suppressor protein have the same asymmetry in strand polarity as the sequenced siRNAs and are imperfect double-stranded RNA duplexes. Moreover, an analysis of siRNAs derived from two other nonrelated positive-strand RNA viruses showed that they displayed the same asymmetry as CymRSV siRNAs. Finally, we show that Tobacco mosaic virus (TMV) carrying a short inverted repeat of the phytoene desaturase (PDS) gene triggered more accumulation of PDS siRNAs than the corresponding antisense PDS sequence. Taken together, these results suggest that virus-derived siRNAs originate predominantly by direct DICER cleavage of imperfect duplexes in the most folded regions of the positive strand of the viral RNA.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10069957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10542148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10570213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10706289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10749213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10850495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-10850496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11157775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11201747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11287229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11452083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11459066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11461699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11485817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11679670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11679672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11701122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11884680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11893497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-11914277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12007417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12016302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12016303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12016304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12045284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12065420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12119378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12142272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12183184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12193644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12198169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12514099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12573220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12600936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12747828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12753592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12805599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12869753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-12941703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-1344890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-14697199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-14704433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-14770011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-14976549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15024409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15102373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15131083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15372041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15372043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15466488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-15469823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-7817878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15919934-8503183
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
79
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7812-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:15919934-Gene Silencing,
pubmed-meshheading:15919934-Oxidoreductases,
pubmed-meshheading:15919934-Plant Diseases,
pubmed-meshheading:15919934-Plant Leaves,
pubmed-meshheading:15919934-RNA, Small Interfering,
pubmed-meshheading:15919934-RNA, Viral,
pubmed-meshheading:15919934-RNA Interference,
pubmed-meshheading:15919934-Ribonuclease III,
pubmed-meshheading:15919934-Tobacco,
pubmed-meshheading:15919934-Tobacco Mosaic Virus,
pubmed-meshheading:15919934-Tombusviridae
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs.
|
|
pubmed:affiliation |
Agricultural Biotechnology Center, Plant Biology Institute, P. O. Box 411, H-2101 Gödöllö, Hungary.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
