Linked Life Data

15917582

Source:http://linkedlifedata.com/resource/pubmed/id/15917582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-5-26
pubmed:abstractText
Various strains of Escherichia coli, isolated from different patients, were screened for type II restriction endonuclease activity. In 1 out of 23 patients, a type II restriction endonuclease activity was found. The restriction endonuclease designated Eco1524I was purified to near homogeneity, based on hydroxyapatite and heparin sepharose chromatography. Eco1524I exhibited endonuclease restriction activity in the pH range from 6.0 to 10.0 (maximum level at pH 8.0) and required Mg2+ as divalent cation. The enzyme was stable till temperature 55 degrees C and pH range from 6.0 to 10.0. Eco1524I recognized the sequence 6-bp palindromic 5'AGG downward arrow CCT 3', producing blunt end and is found to be an isoschizomer of Stu I.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0273-2289
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-99
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Eco1524I, a type II restriction endonuclease: isolation, partial purification, and characterization.
pubmed:affiliation
Laboratoire de Biochimie, Faculté des Sciences de Tunis, Campus Universitaire 1060 Tunis, Tunisia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't