Linked Life Data

15917232

Source:http://linkedlifedata.com/resource/pubmed/id/15917232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2005-7-19
pubmed:abstractText
The Caenorhabditis elegans neuromuscular junction (NMJ) contains three pharmacologically distinct ionotropic receptors: gamma-aminobutyric acid receptors, levamisole-sensitive nicotinic receptors, and levamisole-insensitive nicotinic receptors. The subunit compositions of the gamma-aminobutyric acid- and levamisole-sensitive receptors have been elucidated, but the levamisole-insensitive acetylcholine receptor is uncharacterized. To determine which of the approximately 40 putative nicotinic receptor subunit genes in the C. elegans genome encodes the levamisole-resistant receptor, we utilized MAPCeL, a microarray profiling strategy. Of seven nicotinic receptor subunit transcripts found to be enriched in muscle, five encode the levamisole receptor subunits, leaving two candidates for the levamisole-insensitive receptor: acr-8 and acr-16. Electrophysiological analysis of the acr-16 deletion mutant showed that the levamisole-insensitive muscle acetylcholine current was eliminated, whereas deletion of acr-8 had no effect. These data suggest that ACR-16, like its closest vertebrate homolog, the nicotinic receptor alpha7-subunit, may form homomeric receptors in vivo. Genetic ablation of both the levamisole-sensitive receptor and acr-16 abolished all cholinergic synaptic currents at the NMJ and severely impaired C. elegans locomotion. Therefore, ACR-16-containing receptors account for all non-levamisole-sensitive nicotinic synaptic signaling at the C. elegans NMJ. The determination of subunit composition for all three C. elegans body wall muscle ionotropic receptors provides a critical foundation for future research at this tractable model synapse.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/1 P01 HL6744-01, http://linkedlifedata.com/resource/pubmed/grant/DK58749, http://linkedlifedata.com/resource/pubmed/grant/F31 NS043068, http://linkedlifedata.com/resource/pubmed/grant/F31 NS046923, http://linkedlifedata.com/resource/pubmed/grant/HD15052, http://linkedlifedata.com/resource/pubmed/grant/P01 DK58212, http://linkedlifedata.com/resource/pubmed/grant/P30 CA68485, http://linkedlifedata.com/resource/pubmed/grant/P30 DK58404, http://linkedlifedata.com/resource/pubmed/grant/P30 EY08126, http://linkedlifedata.com/resource/pubmed/grant/P60 DK20593, http://linkedlifedata.com/resource/pubmed/grant/R01 NS041477-01A1, http://linkedlifedata.com/resource/pubmed/grant/R01 NS041477-02, http://linkedlifedata.com/resource/pubmed/grant/R01 NS041477-03, http://linkedlifedata.com/resource/pubmed/grant/R01 NS26115, http://linkedlifedata.com/resource/pubmed/grant/R01 NS41477
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27013-21
pubmed:dateRevised
2011-9-22
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction.
pubmed:affiliation
Department of Biology, University of Illinois, Chicago, Illinois 60607, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural