Source:http://linkedlifedata.com/resource/pubmed/id/15916950
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2005-5-26
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| pubmed:abstractText |
Cilia and flagella play important roles in many physiological processes, including cell and fluid movement, sensory perception, and development. The biogenesis and maintenance of cilia depend on intraflagellar transport (IFT), a motility process that operates bidirectionally along the ciliary axoneme. Disruption in IFT and cilia function causes several human disorders, including polycystic kidneys, retinal dystrophy, neurosensory impairment, and Bardet-Biedl syndrome (BBS). To uncover new ciliary components, including IFT proteins, we compared C. elegans ciliated neuronal and nonciliated cells through serial analysis of gene expression (SAGE) and screened for genes potentially regulated by the ciliogenic transcription factor, DAF-19. Using these complementary approaches, we identified numerous candidate ciliary genes and confirmed the ciliated-cell-specific expression of 14 novel genes. One of these, C27H5.7a, encodes a ciliary protein that undergoes IFT. As with other IFT proteins, its ciliary localization and transport is disrupted by mutations in IFT and bbs genes. Furthermore, we demonstrate that the ciliary structural defect of C. elegans dyf-13(mn396) mutants is caused by a mutation in C27H5.7a. Together, our findings help define a ciliary transcriptome and suggest that DYF-13, an evolutionarily conserved protein, is a novel core IFT component required for cilia function.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DAF-19 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0960-9822
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| pubmed:author |
pubmed-author:BaillieDavid LDL,
pubmed-author:BlacqueOliver EOE,
pubmed-author:BoroevichKeith AKA,
pubmed-author:HoltRob ARA,
pubmed-author:HuangPeterP,
pubmed-author:InglisPeter NPN,
pubmed-author:JonesSteve J MSJ,
pubmed-author:KhattraJaswinderJ,
pubmed-author:LerouxMichel RMR,
pubmed-author:LiChunmeiC,
pubmed-author:MahAllan KAK,
pubmed-author:MarraMarco AMA,
pubmed-author:McKaySheldon JSJ,
pubmed-author:MoermanDonald GDG,
pubmed-author:OuGuangshuoG,
pubmed-author:PerensElliot AEA,
pubmed-author:ShahamShaiS,
pubmed-author:SwobodaPeterP,
pubmed-author:WarnerAdamA
|
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
935-41
|
| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15916950-Animals,
pubmed-meshheading:15916950-Base Sequence,
pubmed-meshheading:15916950-Caenorhabditis elegans,
pubmed-meshheading:15916950-Caenorhabditis elegans Proteins,
pubmed-meshheading:15916950-Cilia,
pubmed-meshheading:15916950-Computational Biology,
pubmed-meshheading:15916950-Gene Expression Profiling,
pubmed-meshheading:15916950-Genomics,
pubmed-meshheading:15916950-Green Fluorescent Proteins,
pubmed-meshheading:15916950-Mutation,
pubmed-meshheading:15916950-Neurons,
pubmed-meshheading:15916950-Protein Transport,
pubmed-meshheading:15916950-Sequence Analysis, DNA,
pubmed-meshheading:15916950-Transcription Factors
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Functional genomics of the cilium, a sensory organelle.
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| pubmed:affiliation |
Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|