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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-5-26
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pubmed:abstractText |
The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5'-triphosphate (ATP), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non-migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relationship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1748-7838
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
371-8
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pubmed:meshHeading |
pubmed-meshheading:15916723-Adenosine Triphosphate,
pubmed-meshheading:15916723-Angiogenesis Inhibitors,
pubmed-meshheading:15916723-Animals,
pubmed-meshheading:15916723-Antineoplastic Agents, Hormonal,
pubmed-meshheading:15916723-Cell Movement,
pubmed-meshheading:15916723-Cell Size,
pubmed-meshheading:15916723-Chloride Channels,
pubmed-meshheading:15916723-Humans,
pubmed-meshheading:15916723-Hypotonic Solutions,
pubmed-meshheading:15916723-Nasopharyngeal Neoplasms,
pubmed-meshheading:15916723-Neoplasm Invasiveness,
pubmed-meshheading:15916723-Nitrobenzoates,
pubmed-meshheading:15916723-Tamoxifen,
pubmed-meshheading:15916723-Tumor Cells, Cultured
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pubmed:year |
2005
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pubmed:articleTitle |
Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells.
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pubmed:affiliation |
Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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