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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017428,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035298,
umls-concept:C0063690,
umls-concept:C0185117,
umls-concept:C0282641,
umls-concept:C0443252,
umls-concept:C1158478,
umls-concept:C1705422,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
2005-5-25
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pubmed:abstractText |
Gene therapy has shown promise in animal models of retinal disease, with the most success achieved to date with viral vectors used for gene delivery. Viral vectors, however, have side effects and limitations and are difficult to manufacture. The present study was conducted in an attempt to develop a novel system for long-term gene transfer in rat retinal pigment epithelium (RPE), by using nonviral transfection methods for gene transfer and the integrase from the bacteriophage phiC31 to confer long-term gene expression by means of genomic integration.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0146-0404
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2140-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15914635-Animals,
pubmed-meshheading:15914635-Bacteriophages,
pubmed-meshheading:15914635-Cell Culture Techniques,
pubmed-meshheading:15914635-Electroporation,
pubmed-meshheading:15914635-Gene Expression,
pubmed-meshheading:15914635-Green Fluorescent Proteins,
pubmed-meshheading:15914635-Integrases,
pubmed-meshheading:15914635-Integration Host Factors,
pubmed-meshheading:15914635-Luciferases,
pubmed-meshheading:15914635-Male,
pubmed-meshheading:15914635-Microscopy, Fluorescence,
pubmed-meshheading:15914635-Pigment Epithelium of Eye,
pubmed-meshheading:15914635-Plasmids,
pubmed-meshheading:15914635-Polymerase Chain Reaction,
pubmed-meshheading:15914635-Rats,
pubmed-meshheading:15914635-Rats, Inbred F344,
pubmed-meshheading:15914635-Transfection,
pubmed-meshheading:15914635-Transgenes
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pubmed:year |
2005
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pubmed:articleTitle |
phiC31 integrase confers genomic integration and long-term transgene expression in rat retina.
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pubmed:affiliation |
Department of Genetics, Stanford University School of Medicine, CA 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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