Linked Life Data

15914187

Source:http://linkedlifedata.com/resource/pubmed/id/15914187

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-5-25
pubmed:abstractText
A piezoelectric immunosensor based on a Nafion membrane interface with a simple immobilization procedure has been developed for the determination of complement C4 in human serum. The polyanionic perfluorosulfonated Nafion polymer was used to modify the electrode surface of quartz-crystal microbalance (QCM) as a platform for the immobilization of complement C4 antibodies. The surface morphology of QCM modified with Nafion membrane was investigated using scanning electron microscopy. The system was optimized with regard to the parameters involved in the preparation of the immunosensor and the assay process. The proposed immunosensor responds well to C4 in the range of 0.08-1.6 microg/ml with a relative standard deviation of <5.3%. Moreover, the proposed immunosensor has advantages in terms of the speed and simplicity of the immobilization procedure as well as the simple and advantageous regeneration process. The experimental results obtained with regard to nonspecific adsorption and recovery indicate that the proposed immunosensor offers a promising alternative tool for the clinical diagnosis of complement C4.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
299
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Piezoelectric immunoassay for complement C4 based on a Nafion-modified interface for antibody immobilization.
pubmed:affiliation
State Key Laboratory of Chem/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies