Linked Life Data

15914083

Source:http://linkedlifedata.com/resource/pubmed/id/15914083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-6-13
pubmed:abstractText
Plant epidermal wax forms a hydrophobic layer covering aerial plant organs which constitutes a barrier against uncontrolled water loss and biotic stresses. Wax biosynthesis requires the coordinated activity of a large number of enzymes for the formation of saturated very-long-chain fatty acids and their further transformation in several aliphatic compounds. We found in the available database 282 candidate genes that may play a role in wax synthesis, regulation and transport. To identify the most interesting candidates, we measured the level of expression of 204 genes in the aerial parts of 15-day-old Arabidopsis seedlings by performing microarray experiments. We showed that only 25% of the putative candidates were expressed to significant levels in our samples, thus significantly reducing the number of genes which will be worth studying using reverse genetics to demonstrate their involvement in wax accumulation. We identified a beta-keto acyl-CoA synthase gene, At5g43760, which is co-regulated with the wax gene CER6 in a number of conditions and organs. By contrast, we showed that neither the fatty acyl-CoA reductase genes nor the wax synthase genes were expressed in 15-day-old leaves and stems, raising questions about the identity of the enzymes involved in the acyl-reduction pathway that accounts for 20% of the total wax amount.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acyltransferases, http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde Oxidoreductases, http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CER protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/CER3 protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/CUT1 protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Starch Synthase, http://linkedlifedata.com/resource/pubmed/chemical/WAX2 protein, Arabidopsis, http://linkedlifedata.com/resource/pubmed/chemical/hexadecanal dehydrogenase..., http://linkedlifedata.com/resource/pubmed/chemical/waxy protein, plant
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
1734
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-58
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Profiling candidate genes involved in wax biosynthesis in Arabidopsis thaliana by microarray analysis.
pubmed:affiliation
Laboratoire de Biogenèse Membranaire, CNRS, UMR 5200, Université Victor Segalen Bordeaux 2, 146 rue léo Saignat, Case 92, 33076 Bordeaux Cedex, France. Patricia.costaglioli@estbb.u-bordeaux2.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't