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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2005-7-12
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pubmed:abstractText |
Increased permeability and reduced cerebral endothelial cell (CEC) viability induced by oxidative stress are the hallmarks of the blood-brain barrier disruption. In our experiments hydrogen peroxide (H2O2, 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in cultured rat CECs. Adrenomedullin (AM) increased TEER, enhanced peripheral localization of F-actin bands and attenuated the increased permeability induced by H2O2. Furthermore, AM treatment preserved mitochondrial membrane potential, attenuated cytochrome c release, and consequently improved CEC viability in H2O2 treated cultures. These results suggest that AM treatment protects CECs against oxidative injury.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0167-0115
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-34
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15913808-Actins,
pubmed-meshheading:15913808-Adrenomedullin,
pubmed-meshheading:15913808-Animals,
pubmed-meshheading:15913808-Blood-Brain Barrier,
pubmed-meshheading:15913808-Brain,
pubmed-meshheading:15913808-Cell Survival,
pubmed-meshheading:15913808-Cells, Cultured,
pubmed-meshheading:15913808-Cytochromes c,
pubmed-meshheading:15913808-Dose-Response Relationship, Drug,
pubmed-meshheading:15913808-Endothelial Cells,
pubmed-meshheading:15913808-Hydrogen Peroxide,
pubmed-meshheading:15913808-Membrane Potentials,
pubmed-meshheading:15913808-Microscopy, Fluorescence,
pubmed-meshheading:15913808-Mitochondria,
pubmed-meshheading:15913808-Oxidants,
pubmed-meshheading:15913808-Oxidative Stress,
pubmed-meshheading:15913808-Peptides,
pubmed-meshheading:15913808-Permeability,
pubmed-meshheading:15913808-Rats,
pubmed-meshheading:15913808-Rats, Wistar,
pubmed-meshheading:15913808-Reperfusion Injury,
pubmed-meshheading:15913808-Telencephalon,
pubmed-meshheading:15913808-Time Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Adrenomedullin protects rat cerebral endothelial cells from oxidant damage in vitro.
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pubmed:affiliation |
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Iseigaoka 1-1, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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