Source:http://linkedlifedata.com/resource/pubmed/id/15913797
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2005-7-18
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| pubmed:abstractText |
The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode filter paper. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by an "in-house" multiplex PCR and a commercial Amplicor HIV-1 PCR test (Roche, version 1.0). HIV-1 RNA qualitative (QL) and quantitative (QT) detection was determined by Nucleic Acid Sequence Based Amplification (NASBA). The average DNA per blood spot recovered from Whatman and S&S IsoCode was not statistically different (p = 0.512) with a range of 218.9+/-46.84 and 225.63+/-88.33 microg, respectively. The concordance of HIV-1 proviral DNA detection by PCR from dried blood spots Whatman and S&S IsoCode was 94% versus 89.4% for sensitivity and 100% versus 100% for specificity. The sensitivity and specificity of HIV-1 RNA QL detection in dried blood spots was 89.7 and 97.5%, respectively. The HIV-1 RNA QT from dried blood spots showed a good correlation in paired dried blood spots and plasma with Pearson correlation, r = 0.817 (R2 = 0.667, P < 0.05). The data showed that dried blood spots could be used for the diagnosis and monitoring of HIV-1 infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0166-0934
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| pubmed:author |
pubmed-author:ChaovavanichAcharaA,
pubmed-author:KuntiranontKaroonK,
pubmed-author:LikanonsakulSiriratS,
pubmed-author:LouisirirotchanakulSudaS,
pubmed-author:SunthornkachitRujaneeR,
pubmed-author:SutthentRuengpungR,
pubmed-author:TanprasertsukSombatS,
pubmed-author:ThiamchaiVipaV,
pubmed-author:UttayamakulSumonmalS
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| pubmed:issnType |
Print
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| pubmed:volume |
128
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
128-34
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15913797-Blood,
pubmed-meshheading:15913797-DNA, Viral,
pubmed-meshheading:15913797-Female,
pubmed-meshheading:15913797-HIV Infections,
pubmed-meshheading:15913797-HIV-1,
pubmed-meshheading:15913797-Humans,
pubmed-meshheading:15913797-Infant,
pubmed-meshheading:15913797-Infant, Newborn,
pubmed-meshheading:15913797-Infectious Disease Transmission, Vertical,
pubmed-meshheading:15913797-Polymerase Chain Reaction,
pubmed-meshheading:15913797-Pregnancy,
pubmed-meshheading:15913797-Pregnancy Complications, Infectious,
pubmed-meshheading:15913797-Proviruses,
pubmed-meshheading:15913797-RNA, Viral,
pubmed-meshheading:15913797-Reagent Kits, Diagnostic,
pubmed-meshheading:15913797-Self-Sustained Sequence Replication,
pubmed-meshheading:15913797-Sensitivity and Specificity,
pubmed-meshheading:15913797-Thailand
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| pubmed:year |
2005
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| pubmed:articleTitle |
Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection.
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| pubmed:affiliation |
National HIV Repository and Bioinformatic Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok 10700, Thailand.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|