Linked Life Data

15913761

Source:http://linkedlifedata.com/resource/pubmed/id/15913761

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
2005-6-16
pubmed:abstractText
To improve the surface biocompatibility of titanium films, a layer-by-layer (LBL) self-assembly technique, based on the polyelectrolyte-mediated electrostatic adsorption of chitosan (Chi) and gelatin (Gel), was used leading to the formation of multilayers on the titanium thin film surfaces. The film growth was initialized by deposition of one layer of positively charged poly(ethylene imine) (PEI). Then the thin film was formed by the alternate deposition of negatively charged Gel and positively charged Chi utilizing electrostatic interactions. The LBL film growth was monitored by several techniques. The chemical composition, surface topography as well as wettability were investigated by using X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM) and water contact angle measurement, respectively. Quantitative XPS analysis showed the alternative change of C/N ratio after four sequential cycles coating of Ti/PEI/Gel/Chi/Gel, which indicated the discrete layer structure of coatings. Uncoated titanium (control sample) displayed a smooth surface morphology (root mean square (RMS) roughness was around 2.5 nm). A full coverage of coating with Gel/Chi layers was achieved on the titanium surface only after the deposition layers of PEI/(Gel/Chi)2. The PEI/Gel/(Chi/Gel)3 layer displayed a rough surface morphology with a tree-like structure (RMS roughness is around 82 nm). These results showed that titanium films could be modified with Chi/Gel which may affect the biocompatibility of the modified titanium films. To confirm this hypothesis, cell proliferation and cell viability of osteoblasts on LBL-modified titanium films as well as control samples were investigated in vitro. The proliferation of osteoblasts on modified titanium films was found to be greater than that on control (p<0.05) after 1 and 7 days culture, respectively. Cell viability measurement showed that the Chi/Gel-modified films have higher cell viability (p<0.05) than the control. These data suggest that Chi/Gel were successfully employed to surface engineer titanium via LBL technique, and enhanced its cell biocompatibility. The approach presented here may be exploited for fabrication of titanium-based implant surfaces.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5960-71
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15913761-Adsorption, pubmed-meshheading:15913761-Biocompatible Materials, pubmed-meshheading:15913761-Cell Line, Tumor, pubmed-meshheading:15913761-Cell Proliferation, pubmed-meshheading:15913761-Cell Survival, pubmed-meshheading:15913761-Chitosan, pubmed-meshheading:15913761-Coated Materials, Biocompatible, pubmed-meshheading:15913761-Gelatin, pubmed-meshheading:15913761-Humans, pubmed-meshheading:15913761-Macromolecular Substances, pubmed-meshheading:15913761-Microscopy, Atomic Force, pubmed-meshheading:15913761-Microscopy, Confocal, pubmed-meshheading:15913761-Microscopy, Electron, Scanning, pubmed-meshheading:15913761-Microscopy, Fluorescence, pubmed-meshheading:15913761-Osteoblasts, pubmed-meshheading:15913761-Polyethyleneimine, pubmed-meshheading:15913761-Polysaccharides, pubmed-meshheading:15913761-Spectrometry, X-Ray Emission, pubmed-meshheading:15913761-Static Electricity, pubmed-meshheading:15913761-Surface Properties, pubmed-meshheading:15913761-Titanium
pubmed:year
2005
pubmed:articleTitle
Polysaccharide-protein surface modification of titanium via a layer-by-layer technique: characterization and cell behaviour aspects.
pubmed:affiliation
Institute of Materials Science & Technology, Friedrich-Schiller-Universität Jena, Löbdergraben 32, Jena 07743, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't