Linked Life Data

15911126

Source:http://linkedlifedata.com/resource/pubmed/id/15911126

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-5-24
pubmed:abstractText
To determine the possible involvement of protease M/neurosin in demyelinating diseases of the CNS, we examined its expression in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE), a recognized animal model of multiple sclerosis (MS). In situ hybridization, immunohistochemistry and quantitative real-time polymerase chain reaction (PCR) demonstrated that EAE caused an increase in the expression of protease M/neurosin mRNA and its protein product throughout the white and gray matter surrounding demyelinating lesions. Combined in situ hybridization and immunohistochemistry demonstrated that most of the cells expressing protease M/neurosin mRNA within control spinal cord showed immunoreactivity for CNPase or NG2, cell-specific markers for oligodendrocytes and their progenitors, respectively. In the spinal cord from mice with EAE, the expression of protease M/neurosin mRNA in CNPase-positive cells appeared to be increased while double-labeled cells positive for protease M/neurosin mRNA and NG2 were rarely found in areas associated with demyelinating lesions. Although a prominent accumulation of inflammatory cells including T-cells was observed in the vicinity of demyelinated lesions, these cells were not associated with protease M/neurosin mRNA expression. The levels of protease M/neurosin mRNA expression were unchanged in the spleen and even decreased in the thymus during the course of EAE. These observations suggest that the differential expression of protease M/neurosin in mature oligodendrocytes and their progenitors is involved in the pathogenesis of MS and EAE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:volume
382
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
82-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15911126-Animals, pubmed-meshheading:15911126-Central Nervous System, pubmed-meshheading:15911126-Encephalomyelitis, Autoimmune, Experimental, pubmed-meshheading:15911126-Female, pubmed-meshheading:15911126-Immunohistochemistry, pubmed-meshheading:15911126-In Situ Hybridization, pubmed-meshheading:15911126-Kallikreins, pubmed-meshheading:15911126-Mice, pubmed-meshheading:15911126-Mice, Inbred C57BL, pubmed-meshheading:15911126-Multiple Sclerosis, pubmed-meshheading:15911126-Myelin Proteins, pubmed-meshheading:15911126-Myelin-Associated Glycoprotein, pubmed-meshheading:15911126-Oligodendroglia, pubmed-meshheading:15911126-RNA, Messenger, pubmed-meshheading:15911126-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15911126-Stem Cells, pubmed-meshheading:15911126-Tumor Necrosis Factor-alpha
pubmed:articleTitle
Differential expression of protease M/neurosin in oligodendrocytes and their progenitors in an animal model of multiple sclerosis.
pubmed:affiliation
Department of Anatomy, Asahikawa Medical College, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510, Japan. rjt001@asahikawa-med.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't