Linked Life Data

15910883

Source:http://linkedlifedata.com/resource/pubmed/id/15910883

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2005-5-24
pubmed:abstractText
The endocannabinoid N-arachidonylethanolamine (AEA) is accumulated by many cell types, but the mechanisms are unknown. Data from several laboratories are consistent with the hypothesis that the accumulation of AEA occurs via the action of a transmembrane carrier that binds and transports AEA. However, other data suggest that AEA is sufficiently lipophilic to transverse plasma membranes by passive diffusion and will accumulate if it is catabolized intracellularly. The controversy is muddied by the use of different cellular models and assays, all of which are assumed to be studying the same phenomena. The purpose of the studies reported herein was: first, to compare AEA accumulation and accumulation inhibitors in cerebellar granule neurons with a glioma cell line; and, second, to compare the neuronal accumulation of AEA with a closely related analog, N-palmitoylethanolamine (PEA). We have found that the accumulation of AEA by neurons and C6 glioma exhibits different affinity for AEA and inhibitor profiles. In addition, we find that the accumulation of AEA and PEA by neurons differs in the amount accumulated and in heterologous inhibition. These studies add to the evidence that the neuronal accumulation of AEA uniquely requires more than passive diffusion and fatty acid amide-mediated catabolism of intracellular AEA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-7064
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1072-8
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Accumulation of anandamide: evidence for cellular diversity.
pubmed:affiliation
Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. chillard@mcw.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural