Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-5-23
pubmed:abstractText
Gamma interferon (IFN-gamma) is a critical cytokine in host defense against salmonella infections, but its role in phagocytic killing of intracellular Salmonella spp. has been investigated mainly in animal rather than human cells. We measured the effect of recombinant IFN-gamma (rIFN-gamma) priming on bacterial internalization, intracellular killing, oxidative burst, and cytokine release during phagocytosis of Salmonella enterica serovar Typhimurium by human monocyte-derived macrophages (MDM). Eleven-day-old MDM, primed for 72 h with rIFN-gamma (100 ng/ml) exhibited an increased proportion of cells with associated bacteria (31% versus 26%, P = 0.036) and a 67% increase in internalized bacteria per cell compared to unprimed cells (P = 0.025). Retrieval of viable bacteria following internalization was reduced 3.6-fold in 72-h primed versus unprimed MDM (interquartile range, 3.1 to 6.4) at 0.5 h due to enhanced early intracellular killing, and this difference was maintained up to 24 h. In contrast, cells primed for only 24 h exhibited no increase in early killing. MDM were competent to produce an early oxidative burst when stimulated with phorbol myristate acetate, which was fully abrogated by the respiratory burst inhibitor diphenyleneiodonium chloride (DPI), but infection of MDM with S. enterica serovar Typhimurium did not cause an increase in the early respiratory burst under unprimed or primed conditions, and DPI had no effect on the early killing of bacteria by primed or unprimed MDM. During 24 h following infection, rIFN-gamma-primed MDM released more interleukin-12 (IL-12) and less IL-10 relative to unprimed cells. We conclude that 72-h priming with rIFN-gamma increases the efficiency of internalization and nonoxidative early intracellular killing of S. enterica serovar Typhimurium by human macrophages and modifies subsequent cytokine release.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-10820271, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-10899909, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11113375, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11166240, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11452125, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11755407, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11929133, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-11992286, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-12004273, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-12117950, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-12244188, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-12874355, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-14734525, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-14985765, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-1730475, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-1827724, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-2492266, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-2838754, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-2957433, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-3523484, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-6411853, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-8188344, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-8406813, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-8606114, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9039773, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9353027, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9393815, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9490634, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9603733, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9746577, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9854038, http://linkedlifedata.com/resource/pubmed/commentcorrection/15908373-9864217
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3445-52
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Gamma interferon enhances internalization and early nonoxidative killing of Salmonella enterica serovar Typhimurium by human macrophages and modifies cytokine responses.
pubmed:affiliation
Wellcome Trust Tropical Centre, Block E, Royal Infirmary Complex, 70 Pembroke Place, Liverpool L69 3GF, UK. magordon@liverpool.ac.uk
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural