Linked Life Data

15908253

Source:http://linkedlifedata.com/resource/pubmed/id/15908253

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9-10
pubmed:dateCreated
2005-9-19
pubmed:abstractText
An understanding of T cell responses that are crucial for control of Mycobacterium tuberculosis (MTB) has major implications for the development of immune-based interventions. We studied the frequency of purified protein derivative (PPD)-specific CD3) cells expressing interleukin-2 (IL)-2, gamma interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha and IL-10 in HIV-negative pulmonary tuberculosis patients (TB, n=30) as well as in healthy individuals (controls, n=21) from Central Africa. Increased frequencies of PPD-stimulated CD3+ cells expressing IL-2, IFN-gamma, and TNF-alpha in TB were seen when compared with frequencies of controls. The presence of type 1 cytokine biased responses in TB patients was supported by a shift in the distribution pattern of cytokine expression from exclusively IL-2 or TNF-alpha expression seen in controls towards an increased frequency of IFN-gamma/IL-2 or IFN-gamma/TNF-alpha co-expression in TB. Higher levels of PPD-induced IFN-gamma in the supernatants from TB patients than from controls were found, which correlated with its intracellular expression. PPD was a weak inducer of IL-10 in T cells and insufficient in promoting cytokine production in TCRgammadelta+CD3+ cells. Non-specific stimulation with PMA and ionomycin revealed increased frequencies of CD4+ cells expressing IFN-gamma in controls, while expression of IL-2, IL-4, IL-10, IL-13, and TNF-alpha was not different. Non-specific cytokine responses of TCRgammadelta+CD3+ cells were similar in all groups. Pulmonary TB in Central Africa is associated with enhanced expression and secretion of specifically induced cytokines that are frequently implicated in host defense against MTB.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1286-4579
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1161-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15908253-Adolescent, pubmed-meshheading:15908253-Adult, pubmed-meshheading:15908253-Aged, pubmed-meshheading:15908253-Antigens, CD3, pubmed-meshheading:15908253-Brefeldin A, pubmed-meshheading:15908253-CD4-Positive T-Lymphocytes, pubmed-meshheading:15908253-Cells, Cultured, pubmed-meshheading:15908253-Cytokines, pubmed-meshheading:15908253-Female, pubmed-meshheading:15908253-Gabon, pubmed-meshheading:15908253-Humans, pubmed-meshheading:15908253-Interferon-gamma, pubmed-meshheading:15908253-Interleukin-10, pubmed-meshheading:15908253-Interleukin-2, pubmed-meshheading:15908253-Ionomycin, pubmed-meshheading:15908253-Lymphocyte Activation, pubmed-meshheading:15908253-Male, pubmed-meshheading:15908253-Middle Aged, pubmed-meshheading:15908253-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:15908253-T-Lymphocyte Subsets, pubmed-meshheading:15908253-Tuberculin, pubmed-meshheading:15908253-Tuberculosis, Pulmonary, pubmed-meshheading:15908253-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Increased specific T cell cytokine responses in patients with active pulmonary tuberculosis from Central Africa.
pubmed:affiliation
Department of Internal Medicine I, Division of Infectious Diseases, Medical University of Vienna, Waehringerguertel 18-20, 090 Vienna, Austria. stefan.winkler@meduniwien.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't