Linked Life Data

15908029

Source:http://linkedlifedata.com/resource/pubmed/id/15908029

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-6-20
pubmed:abstractText
Fed-batch culture techniques were employed to grow Escherichia coli at high-cell densities for the intracellular production of a recombinant rhamnulose 1-phosphate aldolase (RhuA) under the transcriptional control of the strong promoter T5, using a commercially available expression system (E. coli strain M15 and plasmid vector pQE40). A predetermined exponential feeding strategy at constant specific growth rate was selected to maintain carbon source limited growth using a defined medium. Growth rates below 0.36 h(-1) did not cause a severe formation of acetic acid while cell concentration increased exponentially up to 95 g(biomass)l(-1). The intrinsic biomass-substrate yield (gamma(X/S) = 0.48 gg(-1)) and the maintenance coefficient (mS = 0.10 g(substrate)g(biomass)(-1)h(-1)) were calculated from fed-batch growths at different specific growth rates. These values have been employed to determinate the addition profile during the fed-batch growth until IPTG induction, reaching a specific RhuA production levels of 565 AU g(biomass)(-1) which was lower than in batch (1250 AU g(biomass)(-1)). An inverse correlation between volumetric IPTG concentration and specific RhuA activity was found. A correlation between the ratio biomass/OD(600 nm) and the quantity of recombinant protein produced was found. Finally, the proposed process, after optimization of the IPTG concentration, led to significant increase in enzyme concentration and volumetric productivity compared to batch mode (2680 and 1338%, respectively).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-87
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Influence of induction and operation mode on recombinant rhamnulose 1-phosphate aldolase production by Escherichia coli using the T5 promoter.
pubmed:affiliation
Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't